ISSN 2756-3375
African Journal of Immunology Research ISSN 9431-5833 Vol. 7 (1), pp. 001-012, January, 2020. © International Scholars Journals
Full Length Research Paper
Anti-enterovirus 71 activity screening of Taiwanese folk medicinal plants and immune modulation of Ampelopsis brevipedunculata (Maxim.) Trautv against the virus
Hsing-Wen Hsu1, Jen-Hsien Wang2, Chaio-Li Liu2, Chin-Shuh Chen1 and Yuan-Chuen Wang1*
1Department of Food Science and Biotechnology, National Chung Hsing University, 402 Taichung, R.O.C. Taiwan.
2Section of Infectious Diseases, Department of Internal Medicine, China Medical University Hospital, 404 Taichung, R.O.C. Taiwan.
Accepted 13 September, 2019
Abstract
Enterovirus 71 (EV71) infection caused host immune responses and resulted in severe pathogenicity. In this study, a preliminary anti-EV71 activity of 58 Taiwanese folk medicinal plants and further investigations of the anti-EV71 activity and immunomodulatory effect of Ampelopsis brevipedunculata (Maxim.) Trautv (ABT) were performed. Of the 58 plant extracts, 37 extracts exhibited anti-EV71 activity and strong activity was demonstrated by six extracts with average 50% inhibitory concentrations (IC50) below 80 µg/ml. The ABT (one of the most effective plants) acetone extract exhibited relatively high anti-EV71 activity with 26.11 µg/ml of average IC50, 3.6% of that of the positive control (ribavirin, 734.52 µg/ml). The anti-EV71 modes of the ABT extract included viral infection prevention, viral inactivation and anti-viral replication. Acidic pH treatments slightly decreased anti-EV71 activity of the extract. The ABT extract significantly increased the interlukin-6 (IL-6) and IL-1β and decreased the IL-8 levels in the EV71-infected cells. In summary, a high rate of the screened plants exhibited anti-EV71 activity and the greatest activity was demonstrated by ABT. High amounts of IL-6 induction in the infected-cells had high correlation with the anti-viral replication of the ABT extract.
Key words: Antienterovirus 71 activity, Ampelopsis brevipedunculata (Maxim.) Trautv, immune modulation, medicinal plant.