ISSN 2167-0404
Full Length Research Paper
Clinical isolates of Pseudomonas aeruginosa cultured from patients admitted to the Intensive Care Unit (ICU)
Özge Tandoğan Aydoğan, İhsan Çetin and Yağmur Köklükaya
Department of Otolaryngology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
E-mail: Ö[email protected]
Accepted 30 July, 2015
Abstract
Ninety four clinical isolates of Pseudomonas aeruginosa cultured from patients admitted to the Intensive Care Unit (ICU) (N= 37) and Otolaryngology (ORL) (N= 57) during one year (2001-2002) at the Rabta hospital (Tunisia) have been investigated by using serotyping, pyocintyping, drug susceptibility, M13-PCR and PFGE typing. Result shown that most of the isolates at the ICU belonged to serotype O:12 (11/37) that showed high resistance to commonly used antimicrobials ( -lactamins, aminosids, and quinolone) and a predominance for pyocinotype P10. Despite the frequent occurrence of identical serotypes and pyocinotypes, most of the isolates represent unique RAPD-M13 genotype (88/94). PFGE typing detected three distinct clusters amongst the O12 isolates, suggesting a clonal relatedness among multiresistant O12 isolates. This study illustrates the importance of phenotypic and genotypic epidemiological surveillance of predominant O12 serotype clones in such service in local hospital.
Key words: Pseudomonas aeruginosa, epidemiology, clonal diffusion, multidrug resistance.