African Journal of Agriculture and Food Security

ISSN 2375-1177

Table of Contents 2020

Research Article

African Journal of Agriculture and Food Security ISSN 2375-1177 Vol. 8 (3), pp. 001-005, March, 2020. © International Scholars Journals

Full Length Research Paper

Storage influence on the functional properties of malted and unmalted maize (Zea mays L ssp mays) and soybean (Glycine max L Merrill) flour blends

Adetuyi, F.O.*, Badejo, O.F., Ikujenlola, A.V. and Omosuli, S.V.

Department of Food Science and Technology, Rufus Giwa Polytechnic, P.M.B 1019, Owo, Nigeria.

Accepted 20 January 2020

Abstract 

The complementary blends of maize ( Zea mays L ssp mays) and soybean (Glycine max L Merrill) were stored for a period of 12 weeks. The maize grains were malted by subjecting it to cleaning, washing, steeping, sprouting, drying, desprouting, milling and packaging. A portion of the maize flour was blended with soybean flour at the ratio of 70:30 (malted maize flour: soybean flour; unmalted maize flour: soybean flour). The resulting products were stored in polyethylene and plastic containers. The functional properties (Bulk density, viscosity, water and oil absorption capacity, swelling capacity, peroxide value and least gelation concentration) of the products were determined. The result showed that the bulk density and the peroxide value of both malted and unmalted maize flour reduced significantly (P>0.05) when blended with soybean. The swelling capacities of the malted products were lower than those of the unmalted blends and it also decreased though not significantly (P>0.05) with the period of storage. The malting process reduced the viscosity of the products, however; inclusion of soybean flour increased the viscosity. The viscosity and the peroxide value increased significantly (P>0.05) in the two storage containers as the storage period increased. The result revealed that the packaging materials had no significant effects on the parameters assessed.

Key words: Unmalted maize, malted maize, soybean blends, bulk density, swelling capacity, water absorption capacity.

Ikujenlola , S.V. , Adetuyi , Badejo , A.V. and Omosuli, O.F. , F.O.*

Page: 1 - 5

https://doi.org/10.46882/AJAFS/1087

Research Article

African Journal of Agriculture and Food Security ISSN 2375-1177 Vol. 8 (2), pp. 001-016, February, 2019. © International Scholars Journals

Full Length Research Paper

Hazards analysis critical control points (HACCP) and microbiology qualities of sea-foods as affected by handler’s hygience in Ibadan and Lagos, Nigeria

I. O. Okonko1*, O. D. Adejoye2, A. A. Ogun3, A. A. Ogunjobi4, A. O. Nkang1 and B. C. Adebayo-Tayo4

1Department of Virology, University of Ibadan College of Medicine, University College Hospital (UCH), Ibadan, Nigeria. World Health Organization Polio Regional Reference Laboratory, World Health Organization Collaborative Centre for Arbovirus Reference and Research, World Health Organization National Reference Centre for Influenza, HIV Reference Laboratory.

2Department of Biological Sciences, Tai Solarin University of Education, Ijebu-Ode, Nigeria.

3Environmental Health Unit, Department of Epidemiology, Medical Statistics and Environmental Health, Faculty of Public Health, University of Ibadan College of Medicine, University College Hospital (UCH) Ibadan, Nigeria.

4Microbiology and Biotechnology Unit, Department of Botany and Microbiology, University of Ibadan, Ibadan, Nigeria.

Accepted 12 November, 2019

Abstract

This study reports the hazard analysis critical control points (HACCP) and microbiological qualities of seafood products as affected by hygiene of handlers in Ibadan and Lagos, Nigeria. Seafood products were purchased at four different processing plants, two each from Ibadan and Lagos. This study consisted of observing the raw materials, water used, the utensils used and the environment, monitoring all steps of the processing and packaging, recording temperatures during preparation, storage and display and collecting samples of seafood products for total viable counts, coliforms, Salmonella and Shigella counts and fungi counts. A microbiological survey of seafood processors/handlers was also performed. Palms of processors/handlers were swabbed and analyzed for the presence of indicator organisms of sanitary quality. The total viable bacteria count decreased with time from raw to packaging and to when ready for consumption. Coliforms, B. cereus, Staphylococcus aureus , Salmonella sp, and fungi were isolated and had count higher than 103 cells/ml before processing and after processing with few exceptions. A total of 186 organisms were isolated and identified. Almost all palms of the handlers sampled haboured Micrococcus sp. [36(21.1%)] and Enterobacter sp. [30(18.0%)], followed by Bacillus sp. [24 (14.0%)], Flavobacterium sp. [19 (11.1%)], Staphylococcus sp. [17(9.9%)], Escherichia coli [15(8.8%)], Proteus sp [8(4.7%)], Salmonella sp. [7(4.1%)], Citrobacter sp [3(1.8%)], Klebsiella sp [4(2.3%)], Pseudomonas sp. [3(1.8%)], Serratia sp. [3(1.8%)] and Achromobacterium sp [2(1.2%)], Aspergillus formigatus [3(20.0%)], Aspergillus niger [2(13.3%)], Fusarium sp [2(13.3%)], Mucor mucido [3(20.0%)], Neurospora crassa [2(13.3%)] and Rhizopus sp [3(20.0%)]. Seafood processors/handlers may be sources of microbial chance inoculation, microbial food poison, food intoxication and food spoilage hence, processors/handlers may be counter productive by being responsible for public health hazard and loss of revenue. The level of counts appears high for processed and unprocessed products; the presence of coliforms, S. aureus, B. Cereus, Salmonella and fungi showed that processing of these seafood products in a highly contaminated environment and holding at frozen temperature for sale could be risky. Using current WHO/FAO/NAFDAC guidelines and standards for foods and water, none of the food processors/handlers, the utensils and the products were within acceptable standards. There were significant correlations between bacteriological quality and food hygiene training, and waste product management polices. The findings of this study suggests that there is need to improve on hygienic practices as well as HACCP implementation in public food service outlets in order to obtain relatively safe processed seafood products for consumption. The new approach to supervision of food hygiene and sanitary quality, the HACCP system works rationally as it is based on analysis of systematically assembled data on the causes and conditions which evoked the illness of the consumers by food products or meals. Therefore, education of owners of seafood processing plants, processors/handlers on hazards, critical control points and the importance of hygienic environment is imperative. The control measures and monitoring procedures for seafood processing and packaging are thus advocated.

Key words: Coliforms, contamination, frozen seafood, hazards, food hygiene, CCP, control measures, monitoring, microbiological quality, sanitary standards.

O. D. Adejoye, A. A. Ogunjobi, A. O. Nkang and B. C. Adebayo-Tayo, A. A. Ogun, I. O. Okonko*

Page: 1 - 16

https://doi.org/10.46882/AJAFS/1086

Research Article

African Journal of Agriculture and Food Security ISSN 2375-1177 Vol. 8 (2), pp. 001-007, February, 2020. © International Scholars Journals

Full Length Research Paper

Evaluation of physical and sensory properties of Iranian Lavash flat bread supplemented with precipitated whey protein (PWP

Hossein Jooyandeh

Department of Food Science and Technology, Ramin Agricultural and Natural Resources University, Ahvaz, Iran. E-mail: [email protected].

Accepted 11 December, 2019

Abstract

Flat bread is the main staple food for most Iranians and Lavash is commonly consumed flat bread. In this investigation, the effects of addition of precipitated whey protein (PWP) and fermentation time on the physical properties and sensory characteristics of Lavash breads were studied. Dough was prepar-ed with four substitution levels of PWP (25, 50, 75 and 100%, instead of water used for dough making) and fermented at three different fermentation times (30, 60 and 90 min). Increasing level of supple-mentation with PWP generally caused an increase in all sensory scores of Lavash samples. This im-provement was more obvious when fermentation time was increased. Penetrometer values of Lavash samples showed that increasing fermentation time and supplementation up to 75% created significantly softer breads compare to control. In general, increase in the level of supplementation and fermentation time significantly increased bread yellowness (b* value) and redness (a*) and decreased lightness (L* value).

Key words: Flat bread, Lavash, precipitated whey protein, fermentation.

eh , Hossein Jooy

Page: 1 - 7

https://doi.org/10.46882/AJAFS/1085

Research Article

African Journal of Agriculture and Food Security ISSN 2375-1177 Vol. 8 (2), pp. 001-005, February, 2020. © International Scholars Journals

Full Length Research Paper

Physical and nutritive properties of fermented cereal foods

Osungbaro, Taiwo O.

Chemistry Department, the Polytechnic, Ibadan, Nigeria. E-mail: aw_sunbay @yahoo.com. Tel: 08032307645.

Accepted 10 November, 2019

Abstract

Fermented cereal porridge, “Ogi” is made from maize, sorghum or millet. Fermented cereal porridges (and gels) are important staple food items for people of the West African sub- region and are also important weaning foods for infants. The qualities of these foods are measured in terms of their nutritive values evidenced in the biochemical properties and the textural characteristics. These qualities are influenced by many factors of types of cereal grains and the kind of treatments these grains undergo during processing. Many workers seeking to enhance Ogi nutritional status have investigated the ways in which these factors influence its physical and biochemical properties. This review attempts to bring into focus these various efforts.

Key words: Fermented cereal porridges, physical and nutritional properties.

Taiwo O., Osungbaro

Page: 1 - 5

https://doi.org/10.46882/AJAFS/1084

Research Article

African Journal of Agriculture and Food Security ISSN 2375-1177 Vol. 8 (1), pp. 001-012, January, 2020. © International Scholars Journals

Full Length Research Paper

The influence of cultural and physical conditions on the antimicrobial activity of bacteriocin produced by a newly isolated Bacillus megaterium 22 strain

Rowaida Khalil*, Fatima Djadouni, Yasser Elbahloul and Sanaa Omar

Department of Botany, Faculty of Science, Alexandria University, Alexandria, Egypt.

Accepted 06 December, 2019

Abstract

Bacillus megaterium 22, a soil isolate, produced a bacteriocin that exhibited a broad range of inhibitory activity against food-spoilage microorganisms including Salmonella typhimurium and Staphylococcus aureus. The antimicrobial activity peaked at the early stationary phase. De Man Rogosa Shrarpe (MRS) was the best medium for bacteriocin production, where growth of B. megaterium 22 for 12 - 18 h at 30oC, pH 6 - 6.5 resulted in maximum inhibitory effect on the pathogenic indicator strains. Supplemen-tation and/or replacement of medium nutrients demonstrated higher values of bacteriocin activity in the presence of 5 - 10% sucrose, 1% beef extract, and under limited aeration. Bacteriocin activity was significantly stimulated at concentrations of up to 3% NaCl, or 1% KCl. Low levels of spices (curry, red and Black pepper) synergistically stimulated the bacteriocin activity, except for garlic and rosemary where higher concentrations (1%) considerably influenced the activity. The bacteriocin was heat stable for 15 min of exposure to a wide range of temperatures, and over a pH range of 2 - 8 after 1 h of expo-sure. The bacteriocin was stable for up to 30 min of exposure to UV light, and when stored at 4oC for 90 days. The activity was inhibited by proteolytic enzymes and tested organic solvents. SDS-PAGE revealed that the apparent molecular weight of the partially purified bacteriocin ranged from 3.496 to 6.512 kDa. Results presented here support the idea that the bacteriocin may propose some industrial advantages that render it as a good natural food bio preservative candidate.

Key words: Bacillus megaterium, antimicrobial activity, bacteriocin, biopreservation, growth media, spices, storage, indicator strains.

Yasser Elbahloul and Sanaa Omar, Rowaida Khalil*, Fatima Djadouni

Page: 1 - 12

https://doi.org/10.46882/AJAFS/1083

Research Article

African Journal of Agriculture and Food Security ISSN 2375-1177 Vol. 8 (1), pp. 001-006, January, 2020. © International Scholars Journals

Full Length Research Paper

Growth of Lentinus squarrosulus (M.) Singer on sawdust of different tropical tree species

Abbot O. Oghenekaro*, John A. Okhuoya and Emmanuel O. Akpaja

Mushroom Biology Unit, Department of Plant Biology and Biotechnology, University of Benin, P. M. B. 1154, Benin City, Nigeria.

Accepted 14 December, 2019

Abstract

Lentinus squarrosulus (Mont.) Singer was cultivated on the sawdust of five economic tropical tree spe-cies; Chlorophora excelsa, Celtis zenkeri, Guera cedrata, Nesogordenia papaverifera and Brachystegia, collected during processing at the sawmill. The highest yield of fruiting bodies (16.17 ± 1.25 g) was observed on B. nigerica sawdust supplemented with 1% CaCO3, 1% sugar and 20% wheat bran. The lowest yield (4.56 ± 1.84 g), was observed on N. papaverifera sawdust without supple-mentation. The results of this study demonstrate that L. squarrosulus mushroom did not show any particular trend with increase in supplementation level. The study underscores the need for guided use of sawdust types along with the required appropriate supplements.

Key words: Agro-industrial waste, bioconversion, cultivation, edible mushroom, sawmill waste.

John A. Okhuoya and Emmanuel O. Akpaja, Abbot O. Oghenekaro*

Page: 1 - 4

https://doi.org/10.46882/AJAFS/1082