ISSN 2756-3790
Research Article
International Journal of Horticulture and Floriculture ISSN 2756-3790 Vol. 11 (3), pp. 001-007, March, 2023. © International Scholars Journals
Full Length Research Paper
Ecological research on Dobera glabra Forssk. in the Saudi Arabian region of Jazan
I. M. Aref, H. A. El Atta* and A. A. Al Ghtani
Department of Plant Production, King Saud University, P. O. Box 2460, Riyadh 11451, Saudi Arabia.
Accepted 8 October, 2022
Abstract
Research on Dobera glabra Forssk is very little. A study was carried out in Jazan (south western Saudi Arabia) on the ecology of D. glabra. The species was distributed over a large area, but with decreasing density towards the Red Sea coast. Generally, the density of the species is very little. This is combined with 0% survival of seedlings and samplings after 8 months made the species existence endangered and urgent intervention is required to conserve it. Although the frequency of flowering was normal, nevertheless fruiting is extremely little (0.29%) . The species regenerates well with seeds without any pretreatment, however, the seedlings require immediate protection from grazing and other human activities. Seedling growth is extremely slow. All attempts of vegetative propagation of D. glabra from cuttings, failed despite the use of suitable rooting media and growth hormones. Due to the importance of the species as a fodder during the dry season where most other resources are unavailable and as famine food for humans, further research is needed on how to conserve D. glabra.
Key words: Dobera glabra, germination, vegetative propagation, grazing, famine, foods, fodder.
H. A. El Atta* and A. A. Al Ghtani, I. M. Aref
Page: 1 - 7
https://doi.org/10.46882/IJHF/1150Research Article
International Journal of Horticulture and Floriculture ISSN 2756-3790 Vol. 11 (2), pp. 001-005, February, 2023. © International Scholars Journals
Full Length Research Paper
Effect of pre-harvest interventions on the quality and cellulase activity of ber fruit during cold storage
S. K Jawandha*, P. P. S. Gill and Navjot
Department of Horticulture, Punjab Agricultural University, Ludhiana 141004, India.
Accepted 20 December, 2022
Abstract
Studies were carried out to find out the effect of various pre-harvest treatments: CaCl2 (@ 0.5, 1.0 and 2.0%), Ca (NO3)2 (@0.5, 1.0 and 2.0%), GA3 (@ 20, 40 and 60 ppm) and Bavistin (@ 0.1%) on the cellulase activity and quality of 'Umran' ber fruits during cold storage. Marked trees were sprayed at colour break stage with the test chemicals. Fruits were packed in CFB boxes and placed in cold storage (3 - 5°C and 85 - 90% RH) for 30 days. The fruits were evaluated after 10, 20 and 30 days interval for various parameters such as cellulase activity, phenolic content, palatability rating and rotting percentage. Cellulase activity registered a gradual increase up-to 20 days of storage thereafter a decline was noted in all the treatments. The palatability rating increased up to 10 days of storage in all the treatments, except control but subsequently it decreased with longer in storage periods. Among the various pre-harvest treatments CaCl2 (2%) recorded minimum cellulase activity and rotting percentage and registered high palatability rating and phenolics content during cold storage conditions. Studies showed that pre-harvest application of CaCl2 (2%) maintained very good fruit quality and prolonged shelf-life for 20 days under cold storage conditions.
Key words: Ber, storage, cellulase, calcium, GA3.
P. P. S. Gill and Navjot, S. K Jawandha*
Page: 1 - 5
https://doi.org/10.46882/IJHF/1149Research Article
International Journal of Horticulture and Floriculture ISSN 2756-3790 Vol. 11 (1), pp. 001-004, January, 2023. © International Scholars Journals
Full Length Research Paper
Optimization of DNA extraction for ISSR studies in Tectona grandis L.f. - an important forest tree species
C. Narayanan*, Swapnil Dubey, Syed Arif Wali, Nidhi Shukla, Randhir Kumar, A.K. Mandal and S.A. Ansari
Genetics and Plant Propagation Division, Tropical Forest Research Institute, P.O.-R.F.R.C., Mandla Road, Jabalpur – 482 021, India.
Accepted 15 October, 2022
Abstract
Four extraction methods and sample types were evaluated for yield, quality and suitability of genomic DNA for ISSR marker amplification in teak (Tectona grandis). Both CTAB and SDS based extraction procedures extracted better quantity and purity index of genomic DNA. Leaves of trees, bud grafts and seedlings yielded better DNA than seeds. Using identical PCR conditions, DNA extraction methods and sample types influenced amplifications of ISSR markers, with no amplifications among seed samples. DNA extraction method and sample type are very important consideration for reproducible ISSR-based molecular marker analysis in teak.
Key words: DNA extraction, ISSR, CTAB, SDS, teak.
Nidhi Shukla, A.K. Mandal and S.A. Ansari, Randhir Kumar, C. Narayanan*, Swapnil Dubey, Syed Arif Wali
Page: 1 - 4
https://doi.org/10.46882/IJHF/1148Research Article
International Journal of Horticulture and Floriculture ISSN 2756-3790 Vol. 11 (1), pp. 001-005, January, 2023. © International Scholars Journals
Full Length Research Paper
Macro-propagation technique for different physiological ages of Gongronema latifolia Benth cuttings
Agbo, C.U.* and I.U. Obi
Department of Crop Science, University of Nigeria, Nsukka, Nigeria.
Accepted 21 November, 2022
Abstract
Non-wood forest products have been shown to be sustaining the rural poor in Nigeria and other developing countries. They are in most cases threatened to extinction because most species are not domesticated and thus harvested from forests. Macro-propagation of Gongronema latifolia Benth highly valued for its nutritional and medicinal qualities but has not been substantially domesticated was studied using three physiological ages (hardwood, semi-hardwood and soft wood) in Nsukka. Statistical analyses were performed on the attributes of the cuttings and nodal cuts. The study showed that large number of clones could be produced from a single stem cutting of G. latifolia with any of the three physiological ages. Hardwood and semi-hardwood nodal cuts produced five and four shoots/clones respectively and thus have higher potential of producing clones. Macro-propagation technique could thus be utilized to rapidly disseminate selected land races and new varieties of G. latifolia resulting from hybridization.
Key words: Gongronema latifolia, macro-propagation, nodal cuts and physiological age.
C.U.* and I.U. Obi, Agbo
Page: 1 - 5
https://doi.org/10.46882/IJHF/1147Research Article
International Journal of Horticulture and Floriculture ISSN 2756-3790 Vol. 10 (4), pp. 001-004, April, 2022. © International Scholars Journals
Full Length Research Paper
Molecular analysis of dicot-monocot split and relationship among major angiosperm groups
Mir Abid Hussain1, Vijeshver Verma2 and Malik Zanul Abdin3
1SRF (Senior Research Fellow), Biotechnology Division, Indian Institute of Integrative Medicine (CSIR), India.
2Biotechnology Division, Indian Institute of Integrative Medicine, India.
3Department of Biotechnology, Jamia Hamdard, N.Delhi, India.
Accepted 21 December, 2021
Abstract
The relationship among the major angiosperm groups are modeled based on cladistic analyses primarily using RAPD technique. Phylogenetic trees of relationship derived from molecular data confirm dicots as the ancestral class of monocots, there seems no dicot-monocot split. Dicots form an ancestral class of magnoliids and the monocot lineage was derived from one of the basal magnoliids, since monocots share several synapomorphies dicots do not contain all the descendants of their common ancestor.
Key words: RAPD (Random amplified polymorphic DNA), rRNA (Ribosomal RNA), rbcL (Ribulose 1, 5-bisphosphate carboxylase gene).
Vijeshver Verma and Malik Zanul Abdin, Mir Abid Hussain
Page: 1 - 4
https://doi.org/10.46882/IJHF/1141Research Article
International Journal of Horticulture and Floriculture ISSN 2756-3790 Vol. 10 (1), pp. 001-006, January, 2022. © International Scholars Journals
Full Length Research Paper
Assessment of status and biomass of Swertia angustifolia: a high value Himalayan medicinal plant
Arvind Bhatt1*, Anil K Bisht2, R. S. Rawal2 and Uppeandra Dhar2
1School of Biological & Conservation Science, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, South Africa.
2Conservation of Biological Diversity Core, G. B. Pant Institute of Himalayan Environment and Development, Kosi- Katarmal, Almora 263 643 (UA), India.
Accepted 17 September 2021
Abstract
Studies on population assessment and biomass variation in selected populations of Swertia angus-tifolia were undertaken in west Himalaya. Low population density across the surveyed populations indi-cates poor availability of the species in study area. Species showed random distribution and higher fre-quency of occurrence in most of the population, which is the indicator of better potential of the spe-cies in these sites. Density was positively correlated with biomass. The biomass showed peak value in senescence phase and thus has positive consequences of harvesting for the survival of the species.
Key words: Endangered, conservation, Himalaya, Swertia angustifolia, medicinal plant.
R. S. Rawal and Uppeandra Dhar, Arvind Bhatt*, Anil K Bisht
Page: 1 - 6
https://doi.org/10.46882/IJHF/1138