Advances in Agriculture and Agricultural Sciences

Advances in Agriculture and Agricultural Sciences ISSN 2756-326X Vol. 10 (7), pp. 001-006, July, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Genetic purity testing of cotton F1 hybrid DHH-11 and parents revealed by molecular markers

Ashok B. Dongre1, *Mamta P. Raut1, Vishwajit M. Paikrao1 and Sunil S. Pande2

1Biotechnology Laboratory, Central Institute for Cotton Research, Post Box No. 2, Shankar Nagar P.O., Nagpur 440010, Maharashtra, India. 2Rajiv Gandhi Biotechnology centre, RTM Nagpur University, LIT Premises, Amravati Road, Nagpur-440033,MS, India.

Accepted 16 March, 2024

Cotton F1 Hybrid DHH-11 and its parents CPD-420 (male) and CPD-423 (female) was analyzed for the development of markers, identification of Hybrids and ascertaining genetic purity in Gossypium hirsutum L. through PCR based Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSRs) DNA Markers. Twenty RAPD primers and nineteen ISSR primers were used for analysis. RAPD primer, OPA 7 was found to be heteroallelic for parents. OPA 7 identified male specific amplicons of about 350bp in the Hybrid DHH-11. The other repeats of different base pairs were homoallelic for both the parents. ISSR primer, IS 15 was found to be heteroallelic for parents. IS 15 identified a female-specific amplicon of about 1000bp in the Hybrid DHH-11. Comparison of the RAPD and ISSR banding pattern of the parents with respective hybrid clearly identified genuine hybrid. Thus RAPD and ISSR markers individually have their own merits in the identification of parents and their hybrids. However, a combination of two PCR based markers can be used for testing the genetic purity of cotton seeds which will be more reliable substitute for GOT and a tool for seed certification. This study suggested that efficiency of RAPD and ISSR assay was evaluated successfully for hybridity determination, which would be a valuable genomic tool for the cotton breeders.

Keywords: Genetic Purity, Gossypium, RAPD, ISSR.
 

Advances in Agriculture and Agricultural Sciences ISSN 2756-326X Vol. 10 (7), pp. 001-005, July, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Examining Body Dimensions and Live Weight in the Rock Partridge (Alectoris graeca)

Tamer Çağlayan¹*, Kemal Kirikçi¹, Aytekin Günlü² and Sema Alaẟahan¹

¹Department of Animal Science, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey.
²Department of Livestock Economics, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey.

Accepted 21 February, 2024

Abstract

The study was carried out to determine some body measurements of male and female rock partridges of varying age (hatching-14 weeks), and to demonstrate the correlations between these measurements and live weight. Body measurements increased with advancing age. The differences between live weights were significant at 6 and 14th weeks (P<0.05 to 0.01). Shank length and shank width (at the os tarsometatarsus) were found to be greater in males between the 8 and 14th weeks of age (P<0.01). Differences between the two sexes in length of head were significant at 4 and 14th weeks (P<0.05 to 0.01). As of the second week, a high significant correlation (0.366 to 0.751) was determined among all body measurements and live weight (P<0.001). The most decisive body measurements used in the determination of live weight were shank width, head length and shank length. The lowest prediction (R2 12.5%) was determined during the hatching period. However, the highest prediction (R2 = 81.4%) was determined in the 4th week, and generally prediction increased with advancing age (P<0.001). Some body measurements of male and female rock partridges of varying age and the correlation of these measurements with live weight were determined in the study. This study concludes that some body measurements, which can be easily obtained, can be used in the prediction of live weight and as criteria in selection for live weight.

Key words: Partridge, body measurements, live weight, correlation, regression.
 

Advances in Agriculture and Agricultural Sciences ISSN 2756-326X Vol. 10 (6), pp. 001-004, June, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Correlation between Carrot Firmness and Water Content and Total Soluble Solids

Majid Rashidi* and Mohammad Gholami

Department of Agricultural Machinery, Takestan Branch, Islamic Azad University, Takestan, Iran.

Accepted 21 February, 2024

Abstract

Carrot firmness (FIR) is often determined using difficult and time consuming laboratory tests, but it may be more appropriate and economical to develop a method which uses easily available and known quality characteristics of carrot such as water content (WC) and total soluble solids (TSS). In this study, typical two variables linear regression model for predicting FIR of Nantes carrot based on WC and TSS of carrot was suggested. The statistical results of the study indicated that in order to predict carrot FIR based on WC and TSS of carrot, the two variables linear regression model FIR = -2805.8 + 64.9 WC + 36.4 TSS with R2 = 0.84 can be strongly recommended.

Key words: Carrot, quality characteristics, prediction, modeling, firmness, water content, total soluble solids.
 

Advances in Agriculture and Agricultural Sciences ISSN 2756-326X Vol. 10 (5), pp. 001-008, May, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper
    
Real-Time Oil Palm Fruit Maturity Prediction: A Simulation-Based Approach

Mohd. Hudzari Razali¹*, Wan Ishak Wan Ismail^1,2, Abd. Rahman Ramli¹, Md. Nasir Sulaiman³ and Mohd. Haniff B. Harun⁴

¹Intelligent System and Robotics Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, Serdang,
Selangor, Malaysia.
²Department of Biological and Agriculture Engineering, Faculty of Engineering, Universiti Putra Malaysia, Serdang,
Selangor, Malaysia.
³Department of Computer Science and Information Technology, Faculty of Science Computer and Information
Technology, 43400 Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
⁴Crop Physiology, Biological Research Division, Malaysian Palm Oil Board, No. 6, Persiaran Institusi, Bandar Baru
Bangi, 43000 Kajang, Selangor, Malaysia.

Accepted 23 February, 2024

Abstract

This paper is to introduce the new method of procedure in studying the relationship of the mesocarp oil content with the colour skin of oil palm fruit with respect to its maturity. The procedure started after collecting the fruitlets sample from fresh fruit bunches (FFB) during unripe, until overripe stage. The variety of oil palm is PISIFERA (Dura X Tenera). The images on each three age categories of FFB were captured at monitoring area with setting image parameter. NIKON coolpix4500 digital camera with tele converter zooming device was used to capture the image. Subsequently, after the end of the capturing session, the fruitlets were pulled from FFB and sent to analyse for oil mesocarp content. The Soxhlet Extractor Soxhlet apparatus is used to determine the oil content of the fruit based on the experiment of standard bunch analysis procedure. The image will be analysed for optical properties of colour, using developed analysis Graphical User Interface (GUI). Using regression analysis of polynomial 2nd order method, it shows that the optical property of oil palm fruit was significant in determining the oil mesocarp fruit with respect to its degree of maturity.

Key words: Day estimation for harvesting, hue digital value, maturity prediction, mesocarp oil content, oil palm optical properties.

Advances in Agriculture and Agricultural Sciences ISSN 2756-326X Vol. 10 (4), pp. 001-005, April, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Exploring the potential and obstacles of vermiculture practices in Southwest Nigeria

Aladesida, A. A.¹*, Owa, S. O.², Dedeke, G. A.¹ and Adewoyin O. A.³

¹Department of Biological Sciences, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria. *Corresponding author. E-mail: [email protected] or [email protected].
²Department of Biological Sciences, Landmark University, Omu-Aran, Kwara State, Nigeria.
³Department of Zoology, University of Ilorin, Kwara State, Nigeria.    

Received 22 February, 2023; Accepted 26 February, 2023

Abstract

The prospect of vermiculture in the south-western coast of Nigeria was studied. One hundred and two respondents were interviewed in the three coastal towns of Badagry, Epe and Igbokoda. The results show that 94% of respondents made use of earthworms as fish bait, 37% buy their worms and 57% collected by themselves. The respondents, who buy, however, noted that their suppliers do not breed the worms but search for them from marshy areas. The price value placed on the worms ranged between 0.35 and ₦3.10 per kg; while the most quoted prices were 0.35 and ₦0.80 (16.7% each). The average quoted price was ₦0.70 per kg. Forty-six percent (46%) of respondents were willing to buy earthworms if supplied to them; 49% were willing to serve as sales agents if contacted and 66.7% opined that earthworm was their choicest fishing bait. The results indicate positive expectation for vermiculture as a business venture. Vermiculture should, however, put into consideration the production of Alma millsoni and Libyodrilus violaceus, the species quoted as the most preferred and effective for fishing. Vermiculture will go a long way in solving the problem of earthworm scarcity among these people, reduce the stress put on natural populations earthworms, thus conserving the worms and also reduce damage done to the soil environment in the course of excavating for worms.

Key words: Vermiculture, earthworm, prospects.
 

Advances in Agriculture and Agricultural Sciences ISSN 2756-326X Vol. 10 (3), pp. 001-007, March, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Assessment of wheat varieties for resistance to leaf rust in Ethiopian conditions

Netsanet Bacha Hei

Ethiopian Institute of Agricultural Research, Ambo-PPRC, P. O. Box 37, Ambo, Ethiopia. E-mail: [email protected].

Received 5 August, 2023; Accepted 20 September, 2023

Abstract
Leaf rust (Puccinia triticina Eriks) is the most common rust disease of wheat in wheat-producing areas of Ethiopia. The use of cultivars with durable resistance is the most economical way of controlling the disease. Field experiments were conducted at Ambo Plant Protection Research Center, Ethiopia during 2013 to 2014 main cropping seasons to reveal variability for field based slow rusting resistance to leaf rust among 18 improved wheat cultivars grown in Ethiopia. Parameters used as criteria to identify slow rusting included final rust severity (FRS), coefficient of infection (CI), relative area under disease progress curve (rAUDPC) and infection rate (Inf-rate). Among these parameters, FRS, CI and rAUDPC were found to be reliable to assess slow rusting in the cultivars. The results revealed that wheat cultivars Pavon 76, Africa Mayo, Bonny, Galili, Qulqulu, Hawi and Senqegna had low disease severities (< 30%) with moderately susceptible reactions, lower rAUDPC values (>30%) and CI (< 20) and were identified to have good level of slow rusting resistance. Cultivars Kubsa, Galama and PBW 343 had moderate values for slow rusting parameters and were identified as possessing moderate level of slow rusting. The slow rusting cultivars identified from the current study can be used for further manipulation in wheat improvement programs.

Key words: Leaf rust, Puccinia triticina, resistance, slow rusting, wheat.