ISSN 2756-3707
Research Article
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 3 (7), pp. 270-273, July, 2016. © International Scholars Journals
Full Length Research Paper
Impact of intracoronary autologous bone marrow mononuclear cells in patients with dilated cardiomyopathy
P. L. N Kaparthi1*, Gupta Namita2, Lakshmi K. Chelluri2, V. Surya Prakasa Rao1, P. Kantilal Shah1, Adavi Vasantha2, S. Kamaraju Ratnakar2 and K. Ravindhranath1
1Department of Cardiology, Global Hospitals, Lakdi-ka-Pool, Hyderabad –500 004 (A.P), India.
2Department of Transplant Biology and Stem Cell, Global Hospitals, Lakdi-ka-Pool, Hyderabad –500 004 (A.P), India.
*Corresponding author: E-mail: [email protected]. Tel: 9848047521. Fax: 0091-40-2324 4455.
Accepted 05 February, 2016
Abstract
The impact of intracoronary autologous bone marrow mononuclear cells (BMMC) in patients with DCM (dilated cardiomyopathy) with severe left ventricular (LV) dysfunction was assessed. The study included five DCM patients (age group 20 - 65 years) with ejection fraction (EF) of ≤ 30%. Mononuclear cells (Mean MNC count: 9.16 x 107 and Mean CD34 positive cell count: 3.68 x 105) were infused through intracoronary injection into the patients. Left ventricular ejection fraction (LVEF) was increased from 25 30% to 40 - 45% (approximately 500,000 CD34 + cells) in two of the patients. Conclusively, bone marrow mononuclear cell therapy has a potential to be considered a standard therapeutic entity for the treatment of dilated cardiomyopathy with severe LV dysfunction.
Key words: Mononuclear cells, dilated cardiomyopathy, autologous, intra-coronary.
Lakshmi K. Chelluri, P. L. N Kaparthi*, Gupta Namita, Adavi Vasantha, S. Kamaraju Ratnakar and K. Ravindhranath, P. Kantilal Shah, V. Surya Prakasa Rao
Research Article
Evaluation of the microbial attributes of the polluted Alamuyo River on selected surrounding wells
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 3 (8), pp. 274-282, August, 2016. © International Scholars Journals
Full Length Research Paper
Evaluation of the microbial attributes of the polluted Alamuyo River on selected surrounding wells
O. O. Fawole, T. A. Yekeen*, A. A. Ayandele, A. Akinboro, M. A. Azeez and S. O. Adewoye
Department of Pure and Applied Biology, Ladoke Akintola University of Technology, P.M.B. 4000, Ogbomoso, Nigeria.
*Corresponding author. E-mail: [email protected]. Tel: 08036674134.
Accepted 12 March, 2016
Abstract
The physicochemical analysis and microbial load of polluted Alamuyo River in Ibadan, Nigeria and selected wells along its course were evaluated. Toxic effects of water samples obtained from upstream and down stream (F) of the river were also evaluated using Allium cepa root assay. The result of the analysis revealed that the water sample contained toxic substances. However, almost all parameters evaluated were within the allowable limit of treated wastewater. High microbial load was observed in river water samples compared to the well water samples throughout the sampling periods with overall highest value observed at Late Rainy Season upstream water sample (LRS-A). MPN of faecal coliform showed that LDS-A, ERS-F and LRS from A – F were significantly different. Echerichia coli, Bacillus substillis, Staphylococcus aureus, Pseudomonas fragii were among the microbes observed in the samples. T-test analysis on A. cepa root length showed that only 50% of LDS-A, LDS-F and LRS-A were significantly different from control (P < 0.05) though there were reduction in root length in all concentrations tested through out the sampling period except 5% ERSA, 10% ERSF and 10% LRSF. Microscopic evaluation of A. cepa cells showed decreasing number of dividing cells and mitotic indices were not dose dependent in most cases. Chromosome fragments, bridge, lag and disturbed spindle were the aberrations observed in this study. Our result showed that water samples from the river contained toxic substances and high microbial load, which have effects on the surrounding wells due to infiltration.
Key words: Toxicity, Allum cepa, aberration, allowable limits, evaluation, phytotoxic.
O. O. Fawole, A. Akinboro, T. A. Yekeen*, M. A. Azeez and S. O. Adewoye, A. A. Ayandele
Research Article
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 3 (3), pp. 246-249, March, 2016. © International Scholars Journals
Full Length Research Paper
Prevalence of hepatocellular carcinoma in patients infected with and without Hepatitis B virus in Maiduguri, Northeast Nigeria
B. B. Ajayi1, H. A. Nggada2 and A. E. Moses2*
1Department of Immunology, University of Maiduguri Teaching Hospital P. M. B.1414 Maiduguri, Nigeria.
2Department of Histopathology, University of Maiduguri Teaching Hospital P. M. B.1414 Maiduguri, Nigeria.
*Corresponding author. E-mail: [email protected]. Tel: +2348023833116.
Accepted 12 May, 2015
Abstract
Hepatitis B virus (HBV) infection has been reported as one of the aetiological factors for hepatocellular carcinoma (HCCA). This study reports the prevalence of HCCA in patients with and without HBV in Northeast Nigeria over a period of five years. A total of 114 patients consecutively diagnosed with HCCA and tested for Hepatitis B surface Antigen (HBsAg) at the University of Maiduguri Teaching Hospital (UMTH), between January 1996 and December 2000, were studied. There were 81 males and 33 females, ranging in age between 9 months and above 60 years. Of the 114 patients with HCCA, 86.8% were positive for HBsAg of which males accounted for a higher prevalence of 63.1%, while females accounted for 23.7% (2.7:1). HCCA patients from the fouth decade of life and above were observed with a higher prevalence of HBV infection. Among the HBsAg-negative patients with HCCA, no significant gender difference was observed (p > 0.05). However, 13.2% of patients with HCCA were negative for HBsAg. Viral and non-viral aetiologic factors may play a role in developing HCCA in Maiduguri.
Key words: Hepatocellular carcinoma, Hepatitis B virus.
B. B. Ajayi, H. A. Nggada and A. E. Moses*
Research Article
Immunological enhancement of a retrovirus causing non-suppurative chronic arthritis in adult goats
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 3 (5), pp. 258-260, May, 2016. © International Scholars Journals
Full Length Research Paper
Immunological enhancement of a retrovirus causing non-suppurative chronic arthritis in adult goats
Adebayo, I. A.
Department of Animal Production and Health, Federal University of Technology, P. M. B 704, Akure, Nigeria. E-mail: [email protected].
Accepted 19 April, 2015
Abstract
Antibody-mediated enhancement of caprine arthritis encephalitis (CAE) virus in goat synovial membrane (GSM) cells was investigated with regards to time of onset and severity of lesions. Homologous antibody was serially diluted to enhance the growth of the virus as this provided both optimal and subnormal concentrations of the antibody. The cells were incubated for five days and later stained with Giemsa stain. Marked cytopathic effects (CPE) were observed early at sub-optimal concentrations of the antibody indicating that the virus was enhanced at these concentrations. Possible epidemiological implications of these are discussed with relevance to the pathogenesis of retrovirus infections in man and animals.
Key words: CAEV, GSM cells, antibody-mediated enhancement, cytopathic effects.
I. A., Adebayo
Research Article
Establishment of sorghum cell suspension culture system for proteomics studies
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 3 (10), pp. 287-292, October, 2016. © International Scholars Journals
Full Length Research Paper
Establishment of sorghum cell suspension culture system for proteomics studies
Rudo Ngara, Jasper Rees and Bongani K. Ndimba*
Proteomics Research Group, Department of Biotechnology, University of the Western Cape, Private Bag X17, Bellville, 7535, Cape Town, South Africa.
*Corresponding author. E-mail: [email protected]. Tel: +27 21 959 2468. Fax: +27 21 959 1551.
Accepted 07 November, 2015
Abstract
This study describes the establishment of sorghum cell suspension culture system for use in proteomics studies. Friable sorghum callus was initiated from young shoots under completely dark conditions on MS medium supplemented with 3 mg/L 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 2.5 mg/L 1-naphthaleneacetic acid (NAA). Additionally, sorghum cell suspension cultures have been initiated from the friable callus masses in liquid medium with the same composition as the callus initiation medium. Total soluble proteins (TSP) and culture filtrate (CF) proteins were extracted from the cell culture system and solubilised in urea buffer (9 M urea, 2 M thiourea and 4% CHAPS). Both one-dimensional (1D) and two-dimensional (2D) gel analysis of these two proteomes show that the TSP and CF proteomes have different protein expression profiles. The sorghum TSP proteome, which is highly complex, is best resolved when separated on large format, 18 cm, pH 4 - 7 isoelectric focusing (IEF) immobilised pH gradient (IPG) strips. On the other hand, the sorghum CF proteome (secretome) is less complex with most proteins being resolved on mini format, 7 cm, pH 3 - 10 IPG strips. Furthermore, narrowing down the pH range from 3 - 10 to 4 - 7 for the CF proteome resulted in improved protein spot resolution.
Key words: Sorghum, proteomics, callus, cell suspension cultures, total soluble protein, secretome.
Jasper Rees and Bongani K. Ndimba*, Rudo Ngara
Research Article
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 3 (6), pp. 261-269, June, 2016. © International Scholars Journals
Full Length Research Paper
A study of phenolic compounds linked to embryogenic structures induction in cotton cell suspension cultures
Kouakou Tanoh Hilaire1, 2 *, Koné Mongomaké2 Kouadio Yatty Justin2, Amani N’guessan Georges2
1Laboratoire de Mycologie et Biotechnologie Végétale, UFR des Sciences Pharmaceutiques, GESVAB, EA 3675, Université de bordeaux 2, 146 rue Léo-Saignat F-33076 Bordeaux cedex, France.
2Laboratoire de Biologie et d’Amélioration des Productions végétales, UFR Sciences de la Nature, Université d’Abobo-Adjamé, 02 BP 801 Abidjan 02, Côte d’Ivoire.
*Corresponding author. E-mail: [email protected]. Tel: +225 20304200. Fax: +225 20378118.
Accepted 15 July, 2015
Abstract
Trans-resveratrol is a phytoalexin which was found in some grapes and in many other plants. This phenolic compound has gained much attention, as it was indicated to be associated with fungus and disease resistance. Also, it has been shown that phenols compounds could play an important role in somatic embryogenesis induction of various plants. In cotton, embryogenic structures take place in cell suspension cultures. The need of biochemical indicators for the early identification of cells capable to undergo embryogenic structures formation has always been a major concern of researchers. Trans-resveratrol was found only in embryogenic cell suspensions of Coker 312. This phenolic compound started at the first subculture (2.44 µg/g dw) and the maximum level was reached at the third subculture (7.2 µg/g dw) with an increase of nearly 295%. This study showed a correlation between trans-resveratrol synthesis in cotton cell and embryogenic structures induction. trans-resveratrol may be a phenolic indicator for is induction of cotton somatic embryogenesis.
Key words: Trans-resveratrol, suspension culture, embryogenic structures, cotton, Gossypium hirsutum L.
Kouadio Yatty Justin and Amani N’guessan Georges, Koné Mongomaké, Kouakou Tanoh Hilaire *