Advances in Aquaculture and Fisheries Management

Advances in Aquaculture and Fisheries Management ISSN: 9424-2933 Vol. 7 (2), pp. 001-005, February, 2019. © International Scholars Journals

Full Length Research Paper

Generation and affirmation of clones utilizing gynogenesis as a part of Japanese fumble

*Yongxin Yein, Estir I. Vin and Xioang V. E

Department of Fishery Sciences, Faculty of Agriculturen, Xi'an Jiaotong University, Xi'an, China.

Accepted 24 November, 2018

Abstract

Two homozygous clones of Japanese flounder were produced by the gynogenesis that suppresses the cleavage of the eggs in the mitosis and then blocks the extrusion of the second polar body in the meiosis. Microsatellite was used to identify the genetic status of maternal parents and their progenies. 21 polymorphic microsatellite markers were chosen, distributed at the distal region of the 16 linkage group. In this experiment, the two used maternal parents were completely heterozygous, while both mitotic gynogenetic diploids and their clone progenies were homozygous for each marker, demonstrating the successful development of cloning.

Key words: Japanese flounder, microsatellite, clone, gynogenesis.

Advances in Aquaculture and Fisheries Management ISSN: 9424-2933 Vol. 7 (1), pp. 001-007, January, 2019. © International Scholars Journals

Full Length Research Paper

Creation and confirmation of heterozygous clones in Japanese flop, Paralichthys olivaceus by microsatellite marker

*Zhang Yen, Zee Chan and V. E. Zedong

Department of Fishery Sciences, Faculty of Agriculture, Tsinghua University, Beijing, China.

Accepted 12 November, 2018

Abstract

Mitotic gynogenetic diploids Japanese flounder Paralichthys olivaceus were produced by activating eggs with ultra violet (UV) irradiated sperm of red sea bream (Pagrus major), followed by hydrostatic pressure treatment to block the first mitotic division. By crossing two mitotic gynogenetic diploid females with two males, two heterozygous clones of Japanese flounder were produced. Microsatellite marker was used to confirm the genetic status of maternal parents and their progenies. 20 polymorphic microsatellite markers were chosen, covering 16 out of the total 24 linkage groups. The four maternal parents used in this experiment were completely heterozygous, while four mitotic gynogenetic diploids were homozygous for each marker. The genotypes of heterozygous clone progenies were identical and the combination of parental alleles, demonstrates the successful development of cloning.

Key words: Japanese flounder, microsatellite, clone, gynogenesis.

Advances in Aquaculture and Fisheries Management ISSN: 9424-2933 Vol. 7 (1), pp. 001-005, January, 2019. © International Scholars Journals

Full Length Research Paper

The homozygosity confirmation for multiplied haploid Japanese fumble, Paralichthys olivaceus, by microsatellite DNA markers

*Yang Lee, X .Yongxin and Singh Paul

Department of Fishery Sciences, Faculty of Agriculture, Anna University, Chennai, India.

Accepted 19 October, 2018

Abstract

A total of 845 doubled haploids Japanese flounder, Paralichthys olivaceus from three spawns were produced by utilizing hydrostatic pressure treatments on eggs fertilized with ultraviolet (UV)-irradiated sperm of red sea bream (Pagrus major). 481 polymorphic microsatellite markers were used to identify the homozygosity of these doubled haploids, of which, only 31% (265 out of 845) individuals were fully homozygous at all loci tested, while, the rest 580 offspring were homozygous at 183 to 320 loci. Additionally, the embryonic development and external morphology of doubled haploids were also observed and recorded. The whole process of embryonic development was divided into 19 stages based on the morphological characteristics of the developing embryo. Hatching took place 73 to 74 h after fertilization. The means of body weight, body length and body depth in 360 days old fishes were always small in doubled haploids and large in normal diploids, but, the standard deviations and coefficients of variation of doubled haploids were significantly higher than those of normal diploids.

Key words: Doubled haploids, gynogenesis, Japanese flounder, Paralichthys olivaceus.

Advances in Aquaculture and Fisheries Management ISSN: 9424-2933 Vol. 6 (12), pp. 001-007, December, 2018. © International Scholars Journals

Review

Creation and promoting frameworks of cultivated tilapia in China

*Donnie Tsung, Jiang Laozi and Qing Lee

Department of Fisheries and Aquaculture, Faculty of Agriculture, Maharaja Ganga Singh University, Bikaner, India.

Accepted 22 July, 2018

Abstract

Tilapia has become one of the major species of aquatic products in close link with both domestic and international markets. Nowadays, the demand for fish and fisheries product has being exacerbated by the continued increase in human population the world over. This was due to the fact that global captured fishery was declining following the increased human exploitation for the commercially important fishes. Aquaculture or fish farming has become one of the solutions to maintaining the continuous supply of fish to commercial markets. China was by far the largest producer and exporter of tilapia products irrespective of some constraints such as soaring production costs, price and weather fluctuations. In the country, tilapia production was mostly carried out in the southern and southeastern coastal areas where subtropical conditions favored the growth and reproduction of the species. This paper reports on production and marketing systems of tilapia in China. Tilapia products produced in China had to pass through different channels before reaching their final consumers. Major international markets for tilapia products produced in China included among others, the United State, European Union and Russia. This work will guide potential investors and competitors for tilapia market in China and the world at large.

Key words: Tilapia production, marketing system/channels, export, China.

Advances in Aquaculture and Fisheries Management ISSN: 9424-2933 Vol. 6 (11), pp. 001-006, November, 2018. © International Scholars Journals

Full Length Research Paper

Food and feeding habits of the African river prawn (Macrobrachium vollenhovenii, Herklots, 1857) in Epe Lagoon, southwest Nigeria

Abayomi A. Jimoh*, Edwin O. Clarke, Olusegun O. Whenu and Haleemah B. Adeoye

Department of Fishery, Lagos State University, Lagos State, Nigeria.

Accepted 23 July, 2018

Abstract

The study investigated the stomach contents of Macrobrachium vollenhovenii from the commercial artisanal catches in Epe Lagoon in Lagos, southwest Nigeria from July to October 2008 and April to May 2009. The stomach analysis was carried out using frequency of occurrence and numeric methods. It was observed that the prawn fed on a variety of plankton species, which included chlorophyta, euglenophyta, xantophyta, chrysophyta, cladocera, copepoda, protozoa, dinoflagellate, diatoms, insect parts and unidentified food items, with chlorophyta and diatoms forming the most important food items. Chlorophyta constituted 32.00% by number and 83.62% by occurrence, diatoms constituted 31.55% by number and 65.09% by occurrence. Amongst the chlorophyta, Cosmarium granatum was the most preferred, constituting 7.93% by number and 17.67% by occurrence, followed by Ankistrodemus falcatus with 7.57% by number and 16.38% by occurrence, respectively. The least eaten food item was insect parts. The results indicated that M. vollenhovenii can be considered as an omnivorous detritivore.

Key words: Feeding habits, Macrobrachium vollenhovenii, Epe Lagoon.