ISSN 2756-3391
Research Article
African Journal of Parasitology Research ISSN 2756-3391 Vol. 8 (3), pp. 001-008, March, 2021. © International Scholars Journals
Full Length Research Paper
The use of extracts from four local Nigerian plants for the staining of selected bacteria and moulds
W. Braide1*, C. Akobundu1, R. N. Nwaoguikpe2 and L. C. Njiribaeko1
1Department of Microbiology, Federal University of Technology, P. M. B. 1526, Owerri, Imo State, Nigeria.
2Department of Biochemistry, Federal University of Technology, P. M. B. 1526, Owerri, Imo State, Nigeria.
Accepted 20 January, 2021
Abstract
Dyestuffs from four Nigerian plants were extracted and their staining ability on some species of bacteria and moulds were determined. The dyestuffs were extracted using water and organic solvents. Acetic acid treatment of the extracts increased the ability of the extract to stain both bacteria and moulds. Treatment with ammonium hydroxide reduced the staining of the plant extracts except the zobo (Hibuscus sabderiffa) extract. The best staining result was observed with uhe (Pterocarpus osun) extracts. Annato (Bixa orellana) and zobo extracts had moderate effect; whereas Lalle (Lawsonia inermis) showed little or no staining potential. The stains however had greater affinity for moulds than bacteria. The results obtained indicate that extracts with good staining ability have the potential for use in the morphological identification of moulds and in diagnostic bacteriology.
Key words: Plant extracts, staining, moulds, bacteria.
R. N. Nwaoguikpe and L. C. Njiribaeko, W. Braide*, C. Akobundu
Page: 1 - 8
Research Article
African Journal of Parasitology Research ISSN 2756-3391 Vol. 8 (2), pp. 001-007, February, 2021. © International Scholars Journals
Full Length Research Paper
First case of confirmed isosporiasis in an immunocompetent child at a private clinic of Abidjan, Ivory Coast: Case Report
N’da Angbeletchi David AKA1, Kalou Dibert ZIKA2, Gonat Serge Pacôme DOU1, Fatoumata COULIBALY3, Mocket Adolphe EHOUMAN4, Ibrahima KONE5 and Koffi Daho ADOUBRYN6
1University Félix Houphouët-Boigny of Abidjan, 01 BP V34 Abidjan, Cocody.
2Université Alassane Ouattara, BP V18 Bouaké.
3Université Pelero Gon Coulibaly, BP 1328, Korhogo.
4Olopam Pharma and Research & Development, 10 B.P 1274 Abidjan.
5Groupe Médical et de Consulting BOZOUMA, 01 BP 6648 Abidjan.
6Université Alassane Ouattara, BP V18 Bouaké.
Abstract
Accepted 11 January, 2021
Abstract
Isosporiasis is a parasitic disease caused by Isospora belli. Human infection occurs by the ingestion of sporulated oocysts found either in contaminated water, food, or on the hands. We reported here a case of isosporiasis accidentally discovered in a 2-years-old child a day before leaving the hospital for pneumonia. The child had a diarrhoeal episode. Isospora belli oocysts were discovered in his stool samples after microscopic examination. The antibiotic restored the clinical condition of the child after 10 days. The diagnosis of Isoporiasis should be considered in children with digestive disorders in a context of malnutrition or immunocompromised in our settings.
Keywords: Isosporiasis, Cystoisosporiasis, Isospora belli, Immuno-competent Child, Malnutrition, Ivory Coast, Côte d’Ivoire, Africa.
N’da Angbeletchi David AKA, Ibrahima KONE and Koffi Daho ADOUBRYN, Mocket Adolphe EHOUMAN, Kalou Dibert ZIKA, Fatoumata COULIBALY, Gonat Serge Pacôme DOU
Page: 1 - 7
Research Article
African Journal of Parasitology Research ISSN 2756-3391 Vol. 8 (2), pp. 001-007, February, 2021. © International Scholars Journals
Full Length Research Paper
A study of the characterization of a glucose dehydrogenase from Bacillus sp. G3 in Escherichia coli
Xuejiao Chen1,2, Haitao Ding1, Yiqing Du1, Hui Lin1, Zeli Li1 and Yuhua Zhao1*
1Institute of Microbiology, College of Life Science, Zhejiang University, Hangzhou 310058, China.
2Hangzhou Wahaha Group Co., Ltd, Hangzhou 310000, China.
Accepted 17 October, 2020
Abstract
The glucose dehydrogenase gene (gdh), cloned from Bacillus sp. G3, was composed of 786 bp nucleotide and the deduced protein molecular mass of one subunit was 28.1 kDa. The recombinant glucose dehydrogenase (rGDH-G3) was functionally expressed in Escherichia coli. The results revealed that expressed rGDH-G3 had a high specific activity of 371.9 U/mg at 25°C and pH 8.0, with oxidized nicotinamide adenine dinucleotide (NAD+) as the cofactor. The enzyme was optimally active at 40°C and pH 9.0. The enzyme displayed broad specificity for other sugars such as D-galactose or maltose. The catalytic efficiency of the rGDH-G3 would be improved 4 times when oxidized nicotinamide adenine dinucleotide phosphate (NADP+) was used as cofactor instead of NAD+.
Key words: Bacillus sp. G3, enzymatic property, glucose dehydrogenase, inverse polymerase chain reaction (IPCR), optimal pH.
Yiqing Du, Zeli Li and Yuhua Zhao*, Hui Lin, Xuejiao Chen, Haitao Ding
Page: 1 - 7
Research Article
African Journal of Parasitology Research ISSN 2756-3391 Vol. 8 (2), pp. 001-005, February, 2021. © International Scholars Journals
Full Length Research Paper
A study of the molecular epidemiology of nosocomial strains of Acinetobacter baumannii isolated from Nepal
Badri Thapa1, Chanwit Tribuddharat2 and Sulochana Mahat Basnet3
1Department of Microbiology, Kathmandu Medical College, Kathmandu, Nepal, Microbiology Section, Genesis Laboratory and Research, Kathmandu, Nepal.
2Department of Microbiology, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
3Faculty of Health, University of Canberra, Canberra, Australia.
Accepted 22 November, 2020
Abstract
The molecular epidemiology of fifteen clinical strains of Acinetobacter baumannii recovered from various clinical specimens from different wards during January to June, 2010 from a hospital in Nepal was evaluated. Kirby-Bauer disk diffusion test was used for determining in-vitro activities of antibiotics. Molecular epidemiology was investigated by polymerase chain reaction-randomly amplified polymorphic DNA (PCR-RAPD) and plasmid profiling. A. baumannii recovered were multidrug resistant. Isolates represented three antibiotypes (a, b and c). Isolates in antibiotype c (n=12) were resistant to all antibiotics tested while isolates in antibiotype a (n=2) was susceptible to netilmicin and b (n=1) was susceptible to aminoglycosides and fluoroquinolones tested. Four plasmid profiles (i) 1 isolate; (ii) 1 isolate; (iii) 1 isolate; and (iv) 12 isolates and four PCR-RAPD types (I)1isolate; (II) 8 isolates; (III) 1 isolate; (IV) 5 isolates revealed oligoclonal population of A. baumannii. Antibiotypes, plasmid profiles and PCR-RAPD types showed no empirical association. A. baumannii isolates were oligoclonal and multi-drug resistant. The emergence of multi-drug resistant oligoclonal population of this pathogen in a hospital warrants for development of appropriate antibiotic policies and immediate implementation of infection prevention and control measures.
Key words: Acinetobacter baumannii, multidrug-resistant, oligoclonal, Nepal.
Chanwit Tribuddharat and Sulochana Mahat Basnet, Badri Thapa
Page: 1 - 5
Research Article
African Journal of Parasitology Research ISSN 2756-3391 Vol. 8 (2), pp. 001-009, February, 2021. © International Scholars Journals
Full Length Research Paper
Antioxidant and antibacterial activities of Camptotheca acuminate D. seed oil
Lin Wang1, 2, Zhiwei Yang1*, Sicen Wang3, Shuqiu Wang1 and Junxing Liu1*
1School of Basic Medical Sciences, Jiamusi University, Jiamusi 15400, P. R. China.
2The First Affiliated Hospital of Jiamusi University, Jiamusi 154003, P. R. China.
3Research and Engineering Center for Natural Medicine, Xi’an Jiaotong University, Xi’an 710061, P. R. China.
Accepted 21 November, 2020
Abstract
This study was designed to explore the in vitro antioxidant and antibacterial activities of Camptotheca acuminate D. seed oil, which were extracted by supercritical fluid extraction (SFE) or petroleum ether extraction methods. The major constituent of the oil were described as (Z,Z,Z)-9,12,15-Octadecatrien-1-ol (54.92%) and 2-[(trimethylsilyl)oxy]-3-[4-[(trimethylsilyl)oxy]phenyl]-trimethylsilyl ester (26.53%) in supercritical fluid and petroleum ether extracts. The oil and the components were subjected to screen for their possible antioxidant activity by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay and β-carotene bleaching test. In the DPPH test system, free radical scavenging activities of supercritical fluid extracts and petroleum ether extracts were determined to be 7.55 ± 0.11% and 4.38 ± 0.08% (v/v), respectively. As to the β-carotene bleaching test system, the two values were 15.93 ± 0.11% and 6.87 ± 0.15% (v/v), respectively. The activities of antioxidant and antibacterial in components of petroleum ether were more efficient than in components of supercritical fluid extraction. As to the antimicrobial activities of the essential oil against 8 species bacterium, C. acuminate D. seed oil had remarkable antibacterial activity, especially to staphylococcus aureus (ATCC 6538). Thus, C. acuminate D. seed oil could be judged as a kind of patent drug which has antioxidant and antibacterial activity effectively.
Key words: Camptotheca acuminate D. seed oil, antioxidant activities, antibacterial activities.
Lin Wang, Shuqiu Wang and Junxing Liu*, Sicen Wang, Zhiwei Yang*
Page: 1 - 9
Research Article
African Journal of Parasitology Research ISSN 2756-3391 Vol. 8 (1), pp. 001-007, January, 2021. © International Scholars Journals
Short Communication
A case report of an imported cutaneous leishmaniasis in Côte d’Ivoire
N’da Angbeletchi David AKA1, Gonat Serge Pacôme DOU1, Kalou Dibert ZIKA2, Fatoumata COULIBALY3, Mocket Adolphe. EHOUMAN4, Ibrahima KONE5 and Koffi Daho ADOUBRYN2
1University Félix Houphouët-Boigny of Abidjan, 01 BP V34 Abidjan, Cocody.
2Université Alassane Ouattara, BP V18 Bouaké.
3Université Pelero Gon Coulibaly, BP 1328, Korhogo.
4Olopam Pharma and Research & Development, 10 B.P 1274 Abidjan.
5Groupe Médical et de Consulting BOZOUMA, 01 BP 6648 Abidjan.
6Université Alassane Ouattara, BP V18 Bouaké.
Abstract
Accepted 27 December, 2020
Cutaneous leishmaniasis (CL), parasitic and zoonotic disease, is very little known to health professionals in Côte d'Ivoire. It is caused by the bite of a female sandfly that harbors the genus of Leishmania. The species generally involved in Africa are: Leishmania Infantum Nicolle, 1908, Leishmania major Yakimoff & Schokhor, 1914, Leishmania tropica Wright, 1903 and Leishmania aethiopica Bray, Ashford & Bray, 1973. We report here a case of cutaneous leishmaniasis discovered incidentally, presenting involvement of the lower limbs. The patient, a woman, 41-year-old, a housewife, originated from Burkina Faso, who consulted in a private clinic in 2019 for pruriginous skin lesions under a chronic mode. After examination, the disease was diagnosed by positive direct smear, and slide preparations, after scoriations of the skin lesions were carried out and sent to Algeria’s reference center for species determination. Treatment with Metronidazole 500 mg (1tab x3/day for four weeks) permitted to obtain clinical and biological cure without significant side effects. Practitioners should suspect Cutaneous leishmaniasis in case of chronic wounds in endemic zones. The strengthening of human resources, the improvement of our regional laboratories’ technical platform, the integration of the screening and management of leishmaniasis to the program against Buruli ulcer are urgently needed.
Keywords: Leishmaniasis, Cutaneous leishmaniasis, Leishmania, Côte d’Ivoire, Africa, Phlebotomus, Sandyfly, Neglected Tropical Diseases.
N’da Angbeletchi David AKA, Mocket Adolphe. EHOUMAN, Kalou Dibert ZIKA, Gonat Serge Pacôme DOU, Fatoumata COULIBALY, Ibrahima KONE and Koffi Daho ADOUBRYN
Page: 1 - 7