International Journal of Medicine and Medical Sciences

International Journal of Medicine and Medical Sciences ISSN: 2167-0404 Vol. 6 (3), pp. 317-321, March, 2016. © International Scholars Journals

Full Length Research Paper

A study of the characteristics of natural dyes extracted from plants as high dose chemical dosimeters

Siddiqi Salman*, Imtiaz Khalid and Abdul Hussain

Department of Physics, G.C. University, Faisalabad, Punjab, Pakistan.

Accepted 06 December, 2015

Abstract

In the present work aqueous solutions of three natural dyes, that is, Red cabbage, Turmeric and Coriander leaves were irradiated by Cs-137 gamma source in the range of 0-100 kGy. The standard aqueous solutions were scanned by UV/VIS spectrophotometer for the determination of maximum wave length (λ_max) and the absorbance (A) of irradiated samples was measured in UV region. The plot between concentrations (C) and absorbance (A) gave approximate linear relationship and hence verified Beer’s Law. By comparing dosimetric characteristics of all three natural dyes, red cabbage dye was found to be ideal among all. Dosimetric parameters such as effect of concentration and pH values was also studied which proved that these dye solutions are strongly dependent on dye concentration and pH value. Useful dose range for natural dyes to be used as a chemical dosimeter was found to be 60 to 100 kGy.

Key words: Aqueous solutions, natural dye, chemical dosimetry, gamma irradiation, optical density (OD).

International Journal of Medicine and Medical Sciences ISSN: 2167-0404 Vol. 6 (5), pp. 327-331, May, 2016. © International Scholars Journals

Case Report
 
Foreign body (peg pin) in the upper esophagus of Nigerian children: Report of two cases
 
Onotai L.O, Nwogbo A.C, Uyawanne N, Peterside A.
 
Department of Ear, Nose and Throat (ENT) Surgery, University of  Port Harcourt Teaching Hospital (UPTH), Port Harcourt, Rivers State, Nigeria.

Corresponding author. E-mail [email protected]

Received 28 March, 2016; Accepted 13 May, 2016

Abstract
 
Foreign body (FB) ingestion is common in clinical practice especially in children. Its impaction in the upper oesophagus constitutes an important cause of morbidity and mortality in our environment. Due to the availability of various unfriendly household materials and gadgets in our surrounding, children are becoming more prone to the accidental ingestion of unusual foreign bodies such as peg pins. In our geographical area these are the first cases of ingestion of peg pins among children we have encountered in our clinical practice. Hence, we decided to report these cases highlighting the peculiarities of this particular foreign body and the mode of retrieval of the foreign body.
 
Key words: Foreign body, peg pin, upper esophagus, children, Nigeria.
 

International Journal of Medicine and Medical Sciences ISSN: 2167-0404 Vol. 6 (4), pp. 322-326, April, 2016. © International Scholars Journals

Full Length Research Paper 

Electrophoretic behaviour of proteins of Ostertagia ostertagi found in sheep of Kashmir valley

Gurung  Chowdhury*¹, Gurung  Kumar¹ and Chatterjee Roy²

¹Centre of Research for Development, University of Kashmir, Srinagar – 190 006, India.

²Department of Zoology, University of Kashmir, Srinagar-190 006, Kashmir, India.

*Corresponding author. E-mail: gurung_c123 @yahoo.in.

Accepted 04 January, 2016

Abstract

Economic losses due to helminth parasites in sheep throughout the world are considerable. Ostertagia ostertagi is a parasitic cattle nematode belonging to the superfamily of Strongyloidea and the family of Strongylidae. Ostertagia engenders immunity more slowly and is therefore the most important species in older cattle. These are able to induce structural, biochemical and immunological changes in the host like inappetence, diarrhoea, dull hair coat and weight loss, that is, ostertagiosis. At present, control is almost exclusively based on anthelmintics. For isolation of the proteins of the parasite, a well defined methodology was adopted. The abomasae of sheep in which this parasite resides were collected from abattoirs of various districts and were then carried to the laboratory for screening. The parasites were collected in normal saline, washed and stored in 0.05M PBS with pH of 7.4 at 0°C. After refrigeration, frozen nematodes were thawed, homogenized and centrifuged at 1000-15000 rpm for 15 min. The supernatant was thus collected as a protein mixture and stored at -20°C. Protein concentration of the samples was estimated by Lowry method. The samples were then analyzed through Polyacrylamide Gel Electrophoresis (PAGE) and then through Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Protein estimation of the samples was estimated to be 3.9 mg/ml. The processed parasite samples were then subjected to PAGE and SDS-PAGE to determine the presence of the proteins. The proteins were seen as continuous bands intermixing with each other in PAGE analysis. The present study revealed two bands of molecular weights - 67 and 20 kDa in PAGE analysis. The proteins when analyzed through SDS-PAGE were mostly found in the range of 20-70 kDa. The SDS-PAGE analysis showed seven prominent bands of molecular weights of 60, 50, 45, 40, 32, 28 and 20 kDa. The present work was a challenging one since only a single study was conducted in this region on this aspect and thus obviously was a big task to peep into the field where scanty input was available.

Key words: Ostertagia ostertagi, polyacrylamide gel electrophoresis (PAGE), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), PBS.

International Journal of Medicine and Medical Sciences ISSN: 2167-0404 Vol. 6 (2), pp. 311-316, February, 2016. © International Scholars Journals

Case Report

Isolated bilateral thumb arthritis: A bizarre initial presentation of common arthritis

Hussein Abdullah*¹, Khalifa Hamad¹ and Al-Sabah Fahd²

¹Rheumatology Department, King AbdulAziz Medical City, Jeddah, Saudi Arabia.

²Rheumatology Department, King AbdulAziz University Hospital, Jeddah, Saudi Arabia.

Accepted 04 October, 2015

Abstract

Key words: Erosive arthritis, dactylitis, metacarpophalangeal joint arthritis.

International Journal of Medicine and Medical Sciences ISSN 2167-0404 Vol. 5 (11), pp. 299-306, December, 2015. © International Scholars Journals

Full Length Research Paper

Biochemical and hematological diagnostic indices of homozygous sickle cell anemia patients in the steady state

^1*Obimba Kelechukwu Clarence, ²Eziuzor, Samuel Chukwunyelum, ³Ozougwu, Jevas Chibuike, ⁴Ihedimbu, Chiamaka Perpetua

^1*,4Department of Biochemistry, School of Biological Sciences, Federal University of Technology, Owerri, Nigeria.

^2,3Department of Biological Sciences, College of Basic and Applied Sciences, Rhema University Aba, Abia State, Nigeria.

Corresponding author. E-mail: [email protected]

Accepted 12 December, 2015

Abstract

The aim of the study was to investigate the efficacy of the use of some biochemical and hematological clinical indices as diagnostic parameters essential to the treatment and management of homozygous sickle cell anemia in the steady state. Twenty (20) healthy, homozygous AA human subjects and twenty (20) homozygous sickle cell anemia patients in the steady state were subjected to Hemoglobin genotype, packed cell volume (PCV%), white blood cell total (WBC_Total), aspartate amino transferase (AST), serum alkaline phosphatase (AP),  serum triglyceride (TG), serum total cholesterol (C), Total bilirubin (Tb), and Random blood glucose (RBG) analyses. The experimental design is a single factor completely randomized design (CRD). Results recorded of the healthy human subjects and sickle cell anemia patients, expressed as mean ± standard error (S.E) (unit) were as follows : (PCV%) 45 ± 0.1 and 23.87 ± 0.2, (WBC_Total) 5.17 ± 3.94 and 11.27 ± 3.94 (x 10³/μl), (AST) 9.18 ± 1.16 and 19.5 ± 2.16 (U/l), (AP) 140±1.2  and 215.2 ± 1.3 (IU/L), (TG) 75 ± 1 and 117.87 ± 1.2 (mg/dl), (C) 185 ± 2.3 and 140.5 ± 1.2 (mg/dl), (Tb) 0.7 ± 0.1 and 2.8 ± 0.2 (mg%), (RBG) 82.18 ± 4.16  and 90.87± 4.23  (mg/dl), respectively. The mean values of WBC_Total, AST, AP, TG and RBG were significantly higher (p<0.05) : but those of PCV% and C were significantly lower (p<0.05) in sickle cell anemia patients compared with healthy human subjects. Incidence of homozygous sickle cell anemia in the steady state correlated positively and significantly (p<0.05) with significant increase (p<0.05) in WBC_Total, AST, AP, TG and RBG; and significant decrease (p<0.05) in PCV% and C. The statistical regression and correlation between serum triglyceride and PCV% of the patients were significant (p<0.05), r = 0.983. Observed values of PCV% could be used with high precision to predict serum triglyceride levels of homozygous sickle cell anemia patients in the steady state.

Key words: Homozygous, hemoglobin genotype, random blood glucose.