ISSN 2756-3855
Research Article
International Journal of Urology and Nephrology ISSN 2091-1254 Vol. 6 (3), pp. 001-006, March, 2018. © International Scholars Journals
Full Length Research Paper
Penta-and hexapeptide sharing between HPV16 and Homo sapiens proteomes
Darja Kanduc
Department of Biochemistry and Molecular Biology, University of Bari, Italy. E-mail: [email protected], [email protected]. Tel: +39 080 544 3321. Fax: +39 080 544 3321.
Accepted 21 August, 2017
Abstract
The primary sequence of the HPV16 proteome was analyzed for penta- and hexa- peptide sequences shared with human proteins. The following data were obtained: 1) HPV16 and Homo sapiens proteomes share thousands of identical peptide motifs; 2) the overlaps are inter-dispersed among human proteins involved in fundamental housekeeping functions as well as crucial processes such as cell growth, differentiation and neurosensory regulation. The data are discussed in relationship to the potential cross-reactivity risk of an HPV16 vaccine.
Key words: Similarity analysis, sequence-sequence peptide matching, viral versus human proteome over-lapping, vaccine-related cross-reactions.
uc , Darja K
Page: 1 - 6
Research Article
International Journal of Urology and Nephrology ISSN 2091-1254 Vol. 6 (3), pp. 001-008, March, 2018. © International Scholars Journals
Full Length Research paper
Preparation and evaluation of aceclofenac sustained release formulation and comparison of formulated and marketed product
Santanu Ghosh* and B. B. Barik
Utkal University, Department of Pharmaceutical Sciences, Bhubaneswar, Orissa.-751004, India.
Accepted 12 October, 2017
Abstract
The objective of the study was to develop matrix tablets for oral controlled release of aceclofenac. Matrix tablets of aceclofenac, using various viscosity of hydrophilic polymer HPMC in two different proportions, hydrophobic polymer ethyl cellulose and Guar gum were prepared by wet granulation method and subjected to in vitro drug release studies. The drug release from all HPMC matrix tablets followed various release kinetics, formulation no -F7 followed higuchi kinetics. Furthermore, the results of the in vitro studies in pH 7.5 phosphate buffer medium showed that F7 tablets provided controlled release comparable with market sustained release formulation (Aeroff-SR tablets). F7 tablets showed no change in physical appearance, drug content, or in dissolution pattern after storage at 40°C with 75% RH for 6 months. Based on the results of the in vitro studies, it was concluded that the HPMC matrix tablets provided oral controlled release of aceclofenac.
Key words: Aceclofenac, sustained release, matrix tablets, hydroxypropyl methylcellulose.
B. B. Barik, Santanu Ghosh*
Page: 1 - 8
Research Article
International Journal of Urology and Nephrology ISSN 2091-1254 Vol. 6 (2), pp. 001-008, February, 2018. © International Scholars Journals
Full Length Research paper
Effective inhibition of agglutinability of human A and B type erythrocytes by sodium periodate -chloroacetic acid combined treatment
Dipankar Ghosh1*, Susmita Santra1, Sujit Dutta2 and Panchanan Pramanik1
1Department of Chemistry, Indian Institute of Technology, Kharagpur 721302, India.
2Hematology and Blood Bank Division, Kharagpur Sub-divisional Hospital 721302, India.
Accepted 14 September 2017
Abstract
The study focused on effective inhibition of the agglutinability of human A and B type erythrocytes by sodium periodate-chloroacetic acid concerted action. Suppression of agglutinability was determined by using anti-A and anti-B antisera. Effective concentrations of sodium periodate were 6.8 and 2.7 mM, respectively for A and B type erythrocytes in presence of 4.5 mg.ml-1 chloroacetic acid in the reaction mixture. Some major bio-physiological consequences of human erythrocytes including agglutination index, spontaneous red blood cell lysis percentage, zetapotential, morphology, surface functional groups (- OH, > C = O, -COOH) and semi- quantitative oxygen uptake and phagocytic uptake were also analyzed after the chemical treatments. The future work is directed towards the universal human erythrocytes preparation from A and B type human erythrocytes.
Key words: ABH antibodies, agglutination, concerted action, erythrocyte, medicine, transfusion.
Susmita Santra, Sujit Dutta and Panchanan Pramanik, Dipankar Ghosh*
Page: 1 - 8
Case Report
International Journal of Urology and Nephrology ISSN 2091-1254 Vol. 6 (1), pp. 001-003, January, 2018. © International Scholars Journals
Case report
A case of Behcet's disease with abdominal aortic aneurysm
Mansoor Karimifar*, Mansour Salesi, Mozhgan Karimifar and Peyman Mottaghi
Department of Rheumatology, Isfahan University of Medical Sciences, Alzahra Hospital, Soffe Avenue, Isfahan, Iran.
Accepted 14 October, 2017
Abstract
Behcet's disease is a multisystem disease that is mainly classified as vasculitis and may involve many organs like skin, mucosa, eyes, joints, vessels, nervous system, gastrointestinal tract, testis, heart and lungs. This case depicts a rare presentation of Behcet's disease with multiple saccular aneurysms in a 33 year old man. A patient with a persistent pain in the back and flanks with radiation to the testis without a relationship with position and feeding, was admitted in Al-Zahra Hospital, Isfahan. Abdominal and pelvic ultrasonographic evaluation revealed an abdominal aneurysm with thick wall borders, 37 mm in total diameter and 15 mm in open lumen diameter. Spiral computed tomography (CT) of the abdomen and pelvis with contrast revealed aneurysm of the abdominal aorta (4 × 4.5 cm in diameter) beginning below the renal arteries and extending downwardly near the aortic bifurcation. Finally, magnetic resonance angiography (MRA) showed multiple saccular aneurysms of the abdominal aorta at the same places. After repairing the aneurysms by surgical operation, the patient was referred to a rheumatology clinic. After history and physical and laboratory examinations because of recurrent oral aphthae, erythemnodosum, positive result on pathergy test and abdominal aorta aneurysms, a diagnosis of Behcet's disease was made and the patient treated with medical drugs.
Key words: Behcet disease, abdominal aorta aneurysm, vasculitis.
Mansoor Karimifar*, Mozhgan Karimifar and Peyman Mottaghi, Mansour Salesi
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Research Article
International Journal of Urology and Nephrology ISSN 2091-1254 Vol. 6 (2), pp. 001-007, February, 2018. © International Scholars Journals
Full Length Research Paper
Health workers’ knowledge, attitude and behaviour towards hepatitis B infection in Southern Nigeria
S. O. Samuel1*, S. A. Aderibigbe2, T. A. T. Salami3 and O. A. Babatunde2
1Department of Medical Microbiology and Parasitology, Irrua Specialist Teaching Hospital, Edo State, Nigeria.
2Department of Epidemiology and Community Health, University of Ilorin Teaching Hospital, Kwara State, Nigeria.
3Department of Medicine, Irrua Specialist Teaching Hospital, Edo State, Nigeria.
Accepted 07 November, 2017
Abstract
Globally, it is estimated that approximately 400 million individuals are chronic carriers of hepatitis B virus and more than a million people die annually from HBV-related causes. This descriptive cross-sectional study was carried out at Irrua Specialist Teaching Hospital in Edo state, Nigeria, to determine health workers knowledge, attitude and behaviour towards hepatitis B infection using a structured questionnaire. Two hundred respondents from the different cadres of health workers in the hospital were recruited by stratified sampling into the study. More than three-quarters of the respondents (81%) had ever heard of hepatitis B infection prior to the study. Of those that were aware of hepatitis B infection, 92% mentioned blood and blood products as route of transmission of Hepatitis B, 68.5% mentioned needles and sharps while only 37% said that the disease can be transmitted through sexual intercourse. Incorrectly identified routes of transmission by the respondents include faeco-oral transmission (14.2%) and transmission through drinking contaminated water (9.3%). On ways of preventing Hepatitis B infection, correctly identified preventive measures include vaccination by a majority (77.2%) of the respondents. More than three quarters of the respondents (80.9%) said that Hepatitis B can be acquired as a nosocomial infection from the hospital while 69.8% said that Hepatitis B infection is widely transmitted like HIV/AIDS. A little more than three-quarters of the respondents (75.5%), were aware of the existence of Hepatitis B vaccine prior to the study. Only 70.2% have actually ever received Hepatitis B vaccine, out of which only 59.4% completed the vaccination schedule. Poor compliance of Health workers to hepatitis B vaccination is an issue that deserves serious attention. There is a need for health education campaigns for health workers so that they can understand the risks that they are exposed to based on the nature of their work.
Key words: Health workers, hepatitis B, vaccination, knowledge, attitude, behaviour.
T. A. T. Salami and O. A. Babatunde, S. A. Aderibigbe, S. O. Samuel*
Page: 1 - 7
Research Article
International Journal of Urology and Nephrology ISSN 2091-1254 Vol. 5 (6), pp. 185-190, June, 2017. © International Scholars Journals
Full Length Research Paper
Long-term effect of colchicine treatment in preventing urethral stricture recurrence after internal urethrotomy: A Retrospective Trial
1Orhun Sinanoglu, 2Fatih Kurtulus and 3Feride Sinem Akgun
1Maltepe University Department of Urology, Istanbul Turkey.
2Kolan Hospital Groups Urology, İstanbul Turkey Clinic Istanbul Turkey,
3Maltepe University Emergency Department, Istanbul Turkey
Corresponding author. E-mail: [email protected]. Tel. +90 216 444 06 20
Accepted 13 June, 2017
Abstract
Urethral stricture is known to be caused by scar formation leading to urethral lumen stricture in sub-epithelial tissue. It is the most common late complication following transurethral prostate resection (TURP). The aim of this study is to evaluate the efficacy of colchicine treatment in preventing recurrence of urethral stricture in patients after internal urethrotomy, and to determine whether colchicine treatment had a sustained effect in decreasing recurrence of urethral strictures in patients with concomitant diseases. Medical data of patients who had anuria or voiding difficulty and internal urethrotomy in Urology Department of Maltepe University Hospital between January 1, 2011 and December 2016 were collected, and were randomized to colchicine receiver and no-receiver arms. Colchicine was given 1 g/day orally (2x0.5 mg) for two months, and primary efficacy point was defined as whether urethral stricture was developed in 3, 6, and 12 months after internal urethrotomy in both study arms. The study was conducted on 84 males with the mean age of 67.7±7.5 (range= 54-84) years. The mean ages of colchicine receivers and non-receivers were 68.2±7.6 and 67.1±7.6 years, respectively. Recurrence rate of urethral stricture was significantly lower in colchicine receivers (14.6%) than non-receivers (32.6%) (p=0.044 and p<0.05, respectively). In overall evaluation, recurrence rate of urethral stricture was not correlated with an individual concomitant disease, but the rates were increased in the presence of three or four comorbidities. Combination of oral colchicine with internal urethrotomy reduces recurrence rates of urethral stricture recurrence rate significantly.
Keywords: Urethral stricture, colchicine, internal urethrotomy, peyronie disease, male.
Orhun Sinanoglu, Fatih Kurtulus and Feride Sinem Akgun
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