International Journal of Agricultural Sciences

International Journal of Agricultural Sciences ISSN: 2167-0447 Vol. 16 (2), pp. 001-006, February, 2026. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Optimized Rhizome Bud Culture for Accelerated Plantlet Regeneration in Alpinia purpurata

K.P. Kochuthressia¹, S.John Britto¹*, L. Joelri Michael Raj¹, M.O.Jaseentha¹ and S.R.Senthilkumar².

¹The Rapinat Herbarium and Centre for Molecular Systematics, St. Joseph’s College (Autonomous), Tiruchirappalli – 620 002, South India.

²Department of Plant Biology and Plant Biotechnology, St. Joseph’s College (Autonomous), Tiruchirappalli – 620 002, South India.

Accepted 11 November, 2025

A reproducible and efficient protocol is described for multiple shoot induction of Alpinia purpurata using rhizome bud explants. Murashige and Skoog medium supplemented with BA (3.0 mg/l) and Kn (2.0 mg/l) exhibited regeneration rate up to 6.4±0.32 shoots/explants. Spontaneous rooting of shoots occurred in the same concentration of cytokinins. The number of leaves (4.8±0.67) and profuse rooting (8.4±0.70) facilitated 100% of plant recovery on acclimatization. This protocol proves its utility for rapid propagation of A. purpurata to be exploited for pharmaceutical and commercial purposes.

Key words: Alpinia purpurata, micropropagation, rhizome bud culture.
 

International Journal of Agricultural Sciences ISSN: 2167-0447 Vol. 16 (2), pp. 001-010, February, 2026. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

SNP-Based Functional Markers for the Identification of Ve1/Ve2 Resistance Alleles in Tomato Breeding Programs

Ainong Shi1,2, Richard Vierling2, Richard Grazzini3, Pengyin Chen4, Homer Caton1, and Yuejin Weng2

¹Syngenta Seeds, 2369 330th Street, Slater, IA50244, USA;

²Indiana Crop Improvement Association and Department of Agronomy, Purdue University, West Lafayette, IN 47907, USA;

³GardenGenetics LLC, 131 Mendels Way, Bellefonte, PA 16823, USA;

⁴Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, AR 72701, USA.

Accepted 15 October, 2025

Tomato Verticillium wilt, caused by Verticillium dahliae and V. alboatrum is a soil-born fungal disease. Use of host resistance is an effective and environment-friendly method to control this disease. Marker assisted selection (MAS) has become very important and useful in selection of Verticillium wilt resistance genes in tomato (Solanuum lycopersicum L. syn Lycopersicon esculentum Mill.). The objective of this research was to identify Ve gene-derived single nucleotide polymorphism (SNP)markers for MAS in tomato breeding. Six gene-derived SNP markers specific for Ve locus were identified and validated to be useful in selecting Ve resistance gene in different tomato germplasm.

Key words: Single nucleotide polymorphism, Tomato Verticillium wilt; Disease resistance
 

International Journal of Agricultural Sciences ISSN: 2167-0447 Vol. 16 (2), pp. 001-006, February, 2026. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Review

Molecular Insight into Plant Drought Resistance Mechanisms

Mohammad Israil Ansari¹* and Tsan-Piao Lin²

¹Amity Institute of Biotechnology, Amity University, Lucknow Campus, Lucknow, India

²Institute of Plant Biology, National Taiwan University, Taipei, Taiwan

Accepted 2 October, 2025

Plants respond to survive under water-deficit conditions via a series of physiological, cellular, and molecular processes culminating in stress tolerance. Many drought-inducible genes with various functions have been identified by molecular and genomic analyses in various plants, including a number of transcription factors that regulate stress-inducible gene expression. The products of stress-inducible genes function both in the initial stress response and in establishing plant stress tolerance. In this short review, recent progress resulting from analysis of gene expression during the drought-stress response in plants as well as in elucidating the functions of genes implicated in the stress response are summarized.

Keywords: Dehydration, Gene expression, Regulation, Transcription.
 

International Journal of Agricultural Sciences ISSN: 2167-0447 Vol. 16 (2), pp. 001-007, February, 2026. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Utilizing PCR-Based Markers to Evaluate Genetic Stability in Micropropagated Date Palm (cv. Deglet Nour)

A. Othmani1, S. Rhouma1, C. Bayoudh2, R. Mzid4, N. Drira3 and M. Trifi1

1. Laboratoire de Génétique moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, 2092- El- Manar Tunis, Tunisia
2. Laboratoire de Culture in vitro, Centre Régional de Recherches en Agriculture Oasienne, 2260 Degache- Tunisia

3. Laboratoire des Biotechnologies Végétales Appliquées à l’amélioration des cultures, Faculté des Sciences de Sfax,
B.P. 802, 3018 Sfax, Tunisia

4 Laboratoire de physiologie moléculaire des plantes, Centre de Biotechnologie de Borj Cédria, 01 Hammam-Lif.
Tunisia.

Accepted 6 October, 2025

The 2,4-Dichlorophenoxyacetic acid (2,4-D) induced somatic embryogenesis of Tunisian date palm (Phoenix dactylifera L.) cultivar, Deglet Nour and analysis of the true-to-type conformity of the derived plantlets were investigated in this study. For this purpose, two polymerase chain reaction (PCR)-based methods namely, randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) markers were used. Data proved that the modified Murashige and Skoog (MS) media including 1, 10 and 100 mg.l-1 2,4-D have permitted an intensive callogenesis when leaves are incubated in dark. Subcultures on MS medium supplemented with 0.1 mg.l-1 2,4-D stimulated a rapid maturation of somatic embryos in light. A mean of 120 somatic embryos were developed from 0.5 mg callus within 3 months. Embryos germination and conversion to plantlets were successfully achieved after transfer to free plant growth regulators MS medium. On the whole, 75% plantlets survival was established in soil. In addition, RAPD and AFLP analysis were performed in 180 randomly selected plantlets. The resultant DNA banding profiles exhibited a genetic stability of regenerated plantlets compared to the plants of origin. This result strongly supported the true-to-type nature of the in vitro derived plants in date palm.

Keywords: 2,4-D, Date palm, plant regeneration, somatic embryogenesis.
 

International Journal of Agricultural Sciences ISSN: 2167-0447 Vol. 16 (1), pp. 001-007, January, 2026. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Morphological and Anatomical Studies on the Turkish Endemic Gypsophila lepidioides Boiss

Canan Özdemir¹, Mustafa Özkan², Ali Kandemir^3
1Celal Bayar University, Faculty of Science and Art, Department of Biology, Manisa /Turkey.

²Ahi Evran University, Faculty of Science and Art, Department of Biology, Kırşehir/Manisa

³Erzincan University, Education Faculty, Department of Biology, Erzincan/Turkey.

Accepted 9 August 2025

Gypsophila lepidioides Boiss. has a very nice appearance with white flowers, which is a local endemic species for Turkey and categorized as data defficient. The present study is based on the morphological and anatomical investigation of Gypsophila lepidioides. The inflorescence is panicle with many branches, bearing congested, many flowered cymules. The plant taxon was restricted to the gypsum steppe. The anatomical properties of the root, stem and leaves are described. It is determined that the root, stem and leaves are filled with a lot of druse crystals.

Key words: Gypsophila lepidioides, morphology, anatomy, data defficient.
 

International Journal of Agricultural Sciences ISSN: 2167-0447 Vol. 16 (1), pp. 001-009, January, 2026. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Improving Soil Physical Quality through Integrated Tillage and Farmyard Manure Management

Naveed Iqbal Khan¹, Asmat Ullah Malik², Farah Umer³ and M. Irfan Bodla⁴

¹Soil and Water Testing Laboratory, Lahore, Pakistan
²Agronomic Research Station Karor – Layyah, Pakistan ³Soil and Water Testing Laboratory Layyah, Pakistan ⁴University of Agriculture Fasialabad, Pakistan

Accepted 20 September, 2025

A field experiment was conducted at Research Area of Institute of Soil ad Environmental Sciences, University of Agriculture, Faisalabad during year 2004 with the objective to determine the effect of tillage and Farm Yard Manure on soil tilth at sandy clay loam soil. The experiment was laid out in Randomized Complete Blocked Design with four replications having split plot arrangement. Three tillage aspects (Deep tillage (DP), Conventional tillage (CT) and Minimum tillage (MT) and three Farm Yard Manure levels (20 Mg ha-1, 40 Mg ha-1 and control) were used as media of research. The tillage methods were kept in the main plots and FYM levels in subplots. The results revealed that tillage methods significantly increases the soil physical properties as increase in saturated hydraulic conductivity in deep tillage method and decrease in bulk density of soil was noted. The farm yard manure (FYM) also affected the physical properties as it increased saturated hydraulic conductivity. The maximum supply of nitrogen contents (0.079%) was recorded in case of application of FYM (40 Mg ha-1) during minimum tillage.

Keywords: Tillage, FYM, Physical properties, Soil.