International Journal of Enology and Viticulture

International Journal of Enology and Viticulture Vol. 5 (6), pp. 001-005, June, 2018. © International Scholars Journals

Full Length Research Paper

Antibacterial action of an aqueous grape seed polyphenolic extract

Veronica Sanda Chedea^1,5*, Cornelia Braicu², Flore Chirilǎ³, Ciprian Ober⁴ and Carmen Socaciu¹

¹Department of Chemistry and Biochemistry, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur Str., 400372 Cluj-Napoca, Romania.

²Cancer  Institute “I. Chiricuta” Cluj-Napoca, 34-36 Republicii Str., Cluj-Napoca, Romania.

³Depatment of Microbiology-Immunology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur Str., 400372 Cluj-Napoca, Romania.

⁴Department of Anesthesiology and Surgical Propedeutics, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur Str., 400372 Cluj-Napoca, Romania.

⁵Laboratory of Animal Biology, National Research Development Institute for Animal Biology and Nutrition (IBNA), Calea Bucuresti 1, 077015 Balotesti, Romania.

Accepted 25 March, 2018

Abstract

The potential of a polyphenolic grape seed extract for use as a natural antibacterial agent was evaluated. Pure catechin (CS) and a previously LC-MS characterized grape seed phenolic extract (PE) were evaluated as antibacterial agents against ESCHERICHIA COLI and BREVIBACTERIUM LINENS on solid and in liquid culture media. After 48 h incubation on solid medium, PE had a bactericidal effect on the gram positive B. LINENS and a reduction of the microbial growth for E. COLI. The antibacterial agents tested were effective against E. COLI for 13 h, after 7 h incubation, but ineffective against B.LINENS in liquid medium. CS and PE both had an antibacterial effect depending on incubation time.

Key words: Catechin, polyphenolic extract, antibacterial activity, Brevibacterium linens, Escherichia coli B₄₁.

International Journal of Enology and Viticulture Vol. 5 (5), pp. 001-005, May, 2018. © International Scholars Journals

Full Length Research Paper

The study of triploid progenies crossed between different ploidy grapes

Lei Sun, Guo-jun Zhang, Ai-ling Yan and Hai-ying Xu*

Institute of Forestry and Pomology, Beijing Academy of Agricultural and Forestry Science, Beijing, 100093, P. R. China.

Accepted 21 March, 2018

Abstract

The cross between different ploidy grape was one of the effective ways to obtain new seedless cultivars, in this study, through testing the changes of the ovule weight and observing its anatomical structure, the period and mode of ovule abortion in crosses between diploid and tetraploid grape cultivars were studied, reciprocal cross was also conducted between them, the results indicated that the proper sampling period is one of the most important factors of the grape in ovule culture, unlike the seedless cultivars, the embryo abortion did not occur at the same time for most seeded cultivars crossed with different ploidy plants, the ovule abortion was only individual behavior. The period of ovule abortion was correlated with the mature period of the female parent. The abortive embryo of early-maturing cultivars occurred at five weeks after pollination, then the mid-maturing ones and late-maturing ones were at six and nine weeks after pollination comparatively. The reciprocal crossing results showed that this crossing form has great mating obstruction and the situation was more serious when tetraploid cultivars were used as female parent. Few seeds from 2X×4X had good germinability, while those from 4X×2X had lost their germinability and no seedlings were obtained, the highest germination rate and seedling survivals rate were obtained when the ovule was excised 60 days after pollination, it is easier to harvest hybrid progeny when using diploid as female parent.

Key words: Vitis vinifera, ovule culture, hybridization, polyploidy, reciprocal cross.

International Journal of Enology and Viticulture Vol. 5 (5), pp. 001-007, May, 2018. © International Scholars Journals

Full Length Research Paper

A study of suitability of grapevine cultivar Plovdina as a possible indicator plant for flavescence dorée disease

Slobodan Kuzmanović¹*, Dragana Jošić², Žarko Ivanović¹, Tatjana Popović¹, Saša Stojanović¹, Goran Aleksić¹ and Mira Starović¹

¹Institute for Plant Protection and Environment, Belgrade, Serbia.

²Institute for Soil Science, Belgrade, Serbia.

Accepted 21 June, 2017

Abstract

Grapevine cv. Plovdina is a traditional local variety that is very sensitive to flavescence dorée (FD) disease. In this paper, we present the results of top-grafting FD-infected cv. Plovdina plants with scions originating from healthy mother plants of the same cultivar using the "green grafting” method. The first grapevine yellows (GY) symptoms, consisting of yellowing of the Plovdina leaves, have been observed on scions 20 days after grafting. Our results show that GY symptoms were due to phytoplasma and not the viral infection, since grapevine leafroll associated viruses-1, 2 and 3 (GLRaV-1, 2, 3) could not be detected in the scions. Twenty days after-grafting, phytoplasma FD was detected in all grafted scions. Our results indicate that grapevine cv. Plovdina is very suitable as an indicator plant for Flavescence dorée disease.

Key words: Grapevine, phytoplasma flavescence dorée, indicator plant.

International Journal of Enology and Viticulture Vol. 5 (4), pp. 001-005, April, 2018. © International Scholars Journals

Full Length Research Paper

Study of citrus disease prevalence on four citrus varieties at the National Institute of Horticultural Research (NIHORT) Mbato, Okigwe, Imo State, Nigeria

Ezeibekwe, I. O.

Imo State University P. M. B. 2000, Owerri, Imo State, Nigeria. E-mail: [email protected].

Accepted 14 April, 2018

Abstract

The studies were conducted to determine the prevalence of diseases on four varieties of citrus. These are sweet orange (Citrus sinensis), tangelo (Citrus paradise X Citrus reticulate), grape (Citrus paradise) and lemon (Citrus limon). The study showed that, tangelo and sweet oranges were more susceptible to fungal attack than grape and lemon. The disease symptoms commonly observed included dieback on the four species of citrus: tangelo (65%), sweet orange (60%), grape (55%) and lemon (40%). An effective integrated management practice including the use of resistant varieties is recommended.

Key words: Study, citrus, disease, prevalence, Okigwe, Imo State, Nigeria.

International Journal of Enology and Viticulture Vol. 5 (4), pp. 001-008, April, 2018. © International Scholars Journals

Full Length Research Paper

Effects of cluster reduction, herbagreen and humic acid applications on grape yield and quality of Horoz Karasi and Gök üzüm grape cultivars

Aydin Akin

Department of Horticulture, Faculty of Agriculture, University of Selcuk, 42075, Konya, Turkey. E-mail: [email protected]. Tel: +(90) 03322232911. Fax: +(90) 03322410108.

Accepted 16 May, 2017

Abstract

This study was carried out in 5 BB rootstock grafted on Horoz Karasi and Gök üzüm grape varieties (VITIS VINIFERA L.) during the 2010 growth season. Effects of 1/3 cluster reduction (CR), 1/3 CR + herbagreen (HG) and 1/3 CR + humic acid (HA) applications on grape yield and quality of cultivars were examined. The results showed that 1/3 CR + HA application increased grape yield, berry weight, berry red and blue color intensity values of Horoz Karasi grape variety and 1/3 CR application increased grape yield and maturity index values of Gök üzüm grape variety.

Key words: Vitis vinifera L., grape yield, quality, cluster reduction, humic acid, herbagreen.

International Journal of Enology and Viticulture Vol. 5 (3), pp. 001-007, March, 2018. International Scholars Journals

Full Length Research Paper

Effects of some growth regulating applications on leaf yield, raw cellulose and nutrient element content of the Müsküle table grape variety

Aydin Akin

Department of Horticulture, Faculty of Agriculture, University of Selcuk, 42075, Konya, Turkey. E-mail: [email protected]. Tel: +(90) 03322232911. Fax: +(90) 03322410108.

Accepted 25 May, 2017

Abstract

The vine leaf is an important dietary product for human nutrition by virtue of its high nutritious value and low calorie. This study was conducted in the Province of Konya in a vineyard belonging to a producer in the 2010 vegetation period. This study investigated the effects of repetitive applications of herbagreen (HG), humic acid (HA), combined foliar fertilizer (CFF) and HG+CFF performed in the Müẟküle grape variety grafted on 5 BB rootstock on fresh or pickled leaf size and leaf raw cellulose content. HA application increased leaf area and leaf water content values whereas HG+CFF application increased leaf raw cellulose values. The effects of the application on leaf weight and leaf volume were found to be insignificant. It was observed that leaf blade samples were efficient in terms of N, P, Fe, Mn and Cu content, but deficient in K, Ca, Mg and Zn content.

Key words: Mü küle grape leaves, herbagreen, humic acid, combined leaf fertilizer, herbagreen + combined leaf fertilizer, yield, crude fiber content, nutrient values.