African Journal of Virology Research

African Journal of Virology Research ISSN 3421-7347 Vol. 4 (8), pp. 001-003, August, 2010. © International Scholars Journals

Full Length Research Paper

First report of leaf blight of Bakul (Mimusops elengi Linn) caused by Pestalotiopsis clavispora (G.F. Atk.) steyaert in India

¹Lokesh S, ²Vinay B Raghavendra^*, ³Nagesh K S and ⁴M Govindappa

¹Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore- 570 006, Karnataka, India,

²P.G. Department of Biotechnology, Teresian College, Mysore-570011, India,

³Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore- 570 006, Karnataka, India,

⁴Department of Biotechnology, SHridevi Institute of Engineering & Technology, Sira Road, Tumkur-572 106, Karnataka, India.

Accepted 03 January, 2007

Abstract

Pestalotiopsis clavispora (G.F. Atk.) Steyaert was recorded for the first time on Mimusops elengi trees in the University of Mysore campus during 2015-2016. The fungus was isolated from the wilted plant parts and subsequent re-inoculation of the same to healthy plants and its pathogenicity confirmed. Pathogenicity tests showed that Pestalotiopsis clavispora could infect M. elengi, which developed the same symptoms under artificial inoculation conditions to that observed in the field. The fungus was identified based on morphological and culture characteristics as Pestalotiopsis clavispora. Identifications were confirmed using comparisons of DNA sequences of internal transcribed spacers (ITS) regions 1 and 4. This is the first report of Mimusops elengi leaf blight disease caused by P. clavispora.

Keywords: Pestalotiopsis clavispora, Mimusops elengi, internal transcribed spacers (ITS), Pathogenicity.

African Journal of Virology Research ISSN 3421-7347 Vol. 4 (7), pp. 001-006, July, 2010. © International Scholars Journals

Full Length Research Paper

Physiological and nutrition requirements for the determination of Alternaria helianthi in sunflower

Randhi M. E.¹, K. K. Raj² Maharaja E. A.¹ and Nkrimmah Ravi¹

¹Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore- 641 003

²Department of Plant Pathology, Tamil Nadu Agricultural University, Madurai -625 104

Accepted 07 April 2010

Abstract

Ten isolates of Alternaria helianthi were collected from different sunflower growing areas of Tamil Nadu. Most isolates preferred pH levels of 6.96-7.15, temperature 29.22-33.93°C and incubation time of 24 h. The nutritional studies were taken up to know the best source of carbon and nitrogen required for the growth and sporulation of the fungus. Six different carbon sources tried, Glucose was found to be the best source of carbon for the growth and the seven different nitrogen sources tested, ammonium nitrate supported good growth and sporulation.

Key words: Sunflower, Alternaria helianthi, temperature, pH, incubation period, carbon and nitrogen sources.

African Journal of Virology Research ISSN 3421-7347 Vol. 4 (6), pp. 001-007, June, 2010. © International Scholars Journals

Full Length Research Paper

Compatibility nature of Azoxystrobin 25 SC with Pseudomonas fluorescens and Bacillus subtilis on chilli Plants

P. K Mahatma* and Vikrammh R. A

Centre for Plant Protection studies, Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore- 641 003, India.

Accepted 06 August, 2009

Abstract

An experiment was conducted to know the compatibility of Azoxystrobin 25 SC with biocontrol agents by poisoned food technique and turbidity method and with insecticides by emulsion stability test. The biological compatibility was done under glass house condition to find the percent injury. The compatibility showed that Pseudomonas fluorescens and B. subtilis were compatible with Azoxystrobin 25 SC at 5, 10, 50, 100 and 250 ppm, concentration. The physical compatibility studies showed that Azoxystrobin 25 SC was highly compatible commonly used with insecticides viz., Profenphos, Dichlorvos, Monocrotophos, Carbaryl, Dimethoate, Triazophos and Quinalphos. The results from the biological compatibility revealed that insecticides, Dichlorovos and Profenphos were found to be less compatible with Azoxystrobin 25 SC when compared to other insecticides.

Key words: Chilli. Pseudomonas fluorescens, Bacillus subtilis, Azoxystrobin 25 SC, Insecticides.

African Journal of Virology Research ISSN 3421-7347 Vol. 4 (5), pp. 001-012, May, 2010. © International Scholars Journals

Full length Research paper

Effect of seed treatments on disease incidence, seed quality, seed yield, stick yield and fibre yield following line sowing method in CVL-1 variety

S. M. A. Haque¹, I. Hossain² and M. A. Rahman²

¹Senior Scientific Officer, Plant Pathology Department, Pest Management Division, Bangladesh Jute Research Institute, Dhaka-1207, ² Professor, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh.

Accepted 18 April, 2008

Abstract

The present study was conducted to clarify the jute seed storage sustainability using biological agent. The experiments were conducted in the field of JAES and KRS of BJRI and conducted during the period April 2012 to January 2013. Two different seed treatments viz. Provax-200 and BAU- Biofungicide and Seeds having moisture content 9.5% were used for the experiment. Disease incidence occurred lowest at JAES and KRS with BAU- Biofungicide treated seeds were used. BAU- Biofungicide and Provax-200 showed superior means for controlling field fungi with higher seed yield and better improvement of seed quality. The highest disease incidences 20.32% and lowest disease incidences 6.37% were recorded under control and BAU- Biofungicide treated seeds, respectively. Negative relations between disease severity and seed yield, fibre yield and stick yield were observed in seed treatments with BAU- Biofungicide and Provax-200. The highest seed yield (619.58 kg/ha), fibre yield (4.79 ton/ha) and stick yield (10.54 ton/ha) were recorded under BAU- Biofungicide treated seeds and lowest seed yield (461.81 kg/ha), fibre yield (2.87 ton/ha) and stick yield (6.08 ton/ha) were recorded under control treatment. Thus, seed treatment by BAU- Biofungicide enhanced the quality and yield of the jute seed and fibre in the field.

Key Word: Effect, seed treatment, disease incidence, quality, yield, CVL-1.

African Journal of Virology Research ISSN 3421-7347 Vol. 4 (4), pp. 001-004, April, 2010. © International Scholars Journals

Full Length Research Paper

Effects of the Pathogen in Meloidogyne incognita eggs on growth of nematode and sugarbeet reproduction under conservatory conditions

Muhab Abdallah and Ullah Abdula

Plant Pathology Department, Faculty of Agriculture, Helwan University, P.O. Box: 77, Cairo, Egypt.

Accepted 14 March, 2008

Abstract

The impact of three levels of Meloidogyne incognita eggs, that is, 1000, 2000 and 3000 eggs/ plant on plant growth parameters of sugarbeet cv. Nejma and its development and reproduction under greenhouse conditions at 17 ± 5°C was studied. Results indicated that reduction percentage of sugarbeet growth parameters were greatly affected, where the marginal effect was more pronounced on roots than shoots. Moreover, as the inoculation level increased from 1000 up to 3000 eggs of M. incognita /plant, the percentage reduction of plant growth parameters increased, where the highest values were recorded to be 48.97 and 24.18% by 3000 eggs/ plant for shoot dry and whole plant fresh weights, whereas, their lowest values of 30.93 and 4.05% resulted by inoculation level of 1000 eggs/ plant for the same plant growth criteria, respectively. Moreover, the highest rate of nematode reproduction on sugarbeet plants cv. Nejma was recorded by the level of 2000 eggs per plant with value of 1.05 whereas the lowest ones of the same criterion resulted by the levels of 3000 or 1000 eggs per plant that were 0.85 or 0.84, respectively. M. incognita infection to sugar beet plants cv. Nejma at the three levels of egg inoculation obviously reduced N, P, K and total chlorophyll content on leaves of sugarbeet with values of 9.9% (N), 18.5% (P), 8.8% (K), 1.58% (chlorophyll) and 20.0% (N), 27.4% (P), 18.4% (K) and 5.9% (chlorophyll) by 1000 and 3000 eggs/ plant, respectively compared to the uninoculated plant.

Key words: Meloidogyne incognita, sugarbeet, inoculum levels.

African Journal of Virology Research ISSN 3421-7347 Vol. 4 (3), pp. 001-004, March, 2010. © International Scholars Journals

Full Length Research Paper

Screening major diseases of safflower over elite material under field conditions

Rupesh Ashiq and Aarshit Virender

Department of Plant Pathology, Faculty of Agriculture, Anna university, P.O Box 564, Chennai, India.

Accepted 02 March, 2008

Abstract

A field experiment with three replications was conducted at the All India Coordinated Research Project (AICRP) on oilseeds at Marathwada Agricultural University (MAU), Parbhani, Maharashtra on the screening of different elite material against major diseases. Significant differences in resistance to all the diseases were found in the elite material tested. Among the 46 elite lines, 21 and 33 elite lines registered highly resistant reaction against Alternaria leaf spot and to root rot, respectively while 15 lines registered resistant reaction to wilt. This study concludes that screening elite lines for resistance to diseases is an important step in developing varieties/hybrids with improved resistance to different diseases.

Key words: Safflower, diseases, resistance.