International Journal of Medicinal Plants Research

International Journal of Medicinal Plants Research ISSN 2169-303X Vol. 14 (4), pp. 001-009, April, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Evaluation of Antioxidant Activity in Origanum rotundifolium Boiss. from Turkey: Essential Oil and Various Extract

Ismihan Goze1*, Ahmet Alim2, Arzuhan Sihoglu Tepe3, Munevver Sokmen4, Kemal Sevgi3 and Bektas Tepe3

1Vocational School of Health Services, Cumhuriyet University, Sivas 58140, Turkey.
2Public Health Laboratory, Sivas Health Directorate, Sivas 58040, Turkey.
3Cumhuriyet University, Faculty of Science and Literature, Department of Molecular Biology and Genetics, Sivas 58140,
Turkey.
4Karadeniz Technical University, Faculty of Science and Literature, Department of Chemistry, Trabzon 61080, Turkey.

Accepted 05 March, 2025

This study is designed to examine the chemical composition and in vitro antioxidant activity of the essential oil and various extracts of Origanum rotundifolium Boiss. from Turkey. The total of the 40 identified components accounted for 97.23% (GC) and 95.10% (FID) of the total oil. Major components of the oil were borneol, terpinen-4-ol, and spathulenol. To determine the presence of well known and widespread phenolic compounds, water-soluble extract of O. rotundifolium was analyzed by HPLC. In the methanol-soluble extract, caffeic acid, p-coumaric acid, (+)-catechin, and ferulic acid were found in several quantities. Antioxidant activity of the essential oil and extracts of O. rotundifolium has been determined by four different test systems namely DPPH, b-carotene/linoleic acid, chelating effect and reducing power. A strong correlation was observed between the radical scavenging capacity and polarity of the extracts. The methanol-soluble extract which contains the most polar phytochemicals showed the strongest antioxidant effect in all test systems. As expected, amount of the total phenolics and flavonoids was very high in methanol-soluble extract.

Key words: Origanum rotundifolium, extract, essential oil, antioxidant activity, HPLC.
 

International Journal of Medicinal Plants Research ISSN 2169-303X Vol. 14 (3), pp. 001-005, March, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Bioactivity Profiling of Hydnora abyssinica A. Braun Against Diverse Microbial Groups: Fungi and Bacteria

A. M. A. Saadabi* and S. M. H. Ayoub

School of Biotechnology, Faculty of Science and Technology and Faculty of Pharmacy, Al-Neelain University, Khartoum, Sudan.

Accepted 21 March, 2025

Root samples of Hydnora abyssinica were collected from Wad Medani city, central Sudan, to examine their antimicrobial potentials. Root extracts, aqueous, methanolic and chloroform, were obtained and bioassayed in vitro for ability to inhibit growth of 6 human pathogenic fungi and 4 types of bacteria, the differences in bioactivity of aqueous, CHCL3 and MeOH extracts were analyzed. Despite extreme fluctuations in activity, the undiluted aqueous extract was the most inhibitory followed by methanolic extract, while the chloroform extract was the least effective. Growth of pathogens was inhibited by all extracts in a concentration dependent manner. Phytochemical analyses showed preponderance of condensed tannins and phenols in the plant root extracts. The results confirmed the antibacterial and antifungal activity of the roots and support the traditional use of the plant in healing bacterial infections, the possibility of therapeutic use of Sudanese H. abyssinica as antimicrobial agent is worthy of further investigations.

Key words: Hydnora abyssinica, medicinal plants, antibacterial activity, antifungal activity, Sudan.
 

International Journal of Medicinal Plants Research ISSN 2169-303X Vol. 14 (4), pp. 001-005, April, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

In Vitro Mass Propagation of Stevia rebaudiana: A Strategy for Sustainable Production of a Key Medicinal Plant

P. Sairkar*, M. K. Chandravanshi, N. P. Shukla and N. N. Mehrotra

Plant Tissue Culture Laboratory, Centre of Excellence in Biotechnology, M.P. Council of Science and Technology, Bhopal, India.

Accepted 22 February, 2025

Experiments were conducted for standardization of in vitro culture technique of Stevia rebaudiana, an important non-caloric sweetening herb to explore its potential for micro-propagation and callus culture. Nodal segments of the selected herb as explants were cultured for micro-propagation on MS medium containing 0.1 mg/l N6-benzyl amino purine for shoot initiation. Maximum plantlets (83.2 ± 0.445033) were found in MS medium treated with 3.5 mg/l N6-benzyl amino purine at multiplication stage. Young leaves were placed on MS medium containing 2 mg/l 2-4- Dichlorophenoxyacetic acid +1 mg/1 Kinetin was given best result of callusing. Higher regeneration of plantlets (3.8 plantlets/calli) was obtained by placing callus on MS medium with 5 mg/l BA + 1 mg/l NA. Highest rooting average (11.1 ± 0.264052) was recorded on 1/2MS medium with 100 mg/l activated charcoal. The rooted plantlets were hardened in 1:1:1 ratio of sand: soil: vermicompost and successfully established in soil.

Key words: Stevia rebaudiana, in vitro propagation, regeneration of callus, non-caloric sweetener, medicinal plant, N6-benzyl amino purine, 2, 4-Dichlorophenoxyacetic acid.
 

International Journal of Medicinal Plants Research ISSN 2169-303X Vol. 14 (3), pp. 001-005, March, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

The Effects of Herbal-Marine Compound MS14 on Quality of Life in Multiple Sclerosis: A Randomized Placebo-Controlled Crossover Trial

Mohsen Naseri1, Amrollah Ahmadi2, Kourosh Gharegozli3, Masoud Nabavi4, Soghrat Faghihzadeh5, Nevoid Ashtarian6, Fariba Montazami6 and Hossein Rezaeizadeh7

1Shahed Medicinal Plants Research Center, Head of Department of Traditional Iranian Medicine, Shahed University, P.
O. Box: 13185-1678, Tehran, Iran.
2Traditional Iranian Medicine Research Center, Shahed University, P. O. Box: 13185-1678, Tehran, Iran.
3Department of Neurology, Shahid Beheshti University of Medical Science, P. O. Box 13185 - 1678, Tehran, Iran.
4Deparment of Neurology, Faculty of Medicine, Shahed University, P. O. Box: 13185-1678, Tehran, Iran.
5Deparment of Biostatistics, Faculty of Medicine, Tarbiat Modarres University, P. O. Box: 13185-1678, Tehran, Iran.
6Loghman Hospital, Shahid Beheshti University of Medical Science, P. O. Box 13185 -1678, Tehran, Iran.
7Student's Scientific Research Center, Medical Sciences/University of Tehran, P. O. Box: 13185-1678, Tehran, Iran.

Accepted 21 January, 2025

Current research into new drugs is focused on agents that will change multiple sclerosis process. MS14 is an herbal-marine product, based on anecdotal evidences in traditional Iranian medicine (TIM). The objective of this study was to assess the safety and efficacy of MS14 in volunteer patients. This study was a randomized placebo -controlled, double blind, crossover clinical trial in patients who had not responded to common MS drug regimens. Common toxicity criteria and quality of life (QOL) ques-tionnaire were assessed. No significant changes were observed in vital signs, biochemical, hema-tological, liver and kidney function tests. Moreover, the improvement of patients' mobility (lower limb) was significant, comparison before treatment and placebo group (p-value: 0.048). These results suggest the potential benefits of MS14 on QOL in patients and attenuating and remodeling of multiple sclerosis disease and although the results confirm the safety and relative efficacy of MS14.

Key words: Multiple sclerosis, MS14, Iranian traditional medicine.
 

International Journal of Medicinal Plants Research ISSN 2169-303X Vol. 14 (3), pp. 001-006, March, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Characterization and Standardization of Niprisan: A Herbal Remedy for Sickle Cell Disease

Obodozie O. O., Ameh S. J.*, Afolabi E. K., Oyedele E.O., Ache T. A., Onanuga C. E., Ibe M. C.

and Inyang U. S.

Department of Medicinal Chemistry and Quality Control, National Institute for Pharmaceutical Research and Development (NIPRD), PMB 21, Garki, Idu Industrial Area, Abuja, Federal Capital Territory, Nigeria.

Accepted 21 January, 2025

Niprisan is a dry extract preparation of five components: the seeds of Piper guineense; flower buds of Eugenia caryophyllata; stem parts of Pterocarpus osun; leaf stalk of Sorghum bicolor and trona. The product was developed in our Institute from a traditional recipe used in Nigeria for treating sickle cell crises. This study aims to provide for the components, the following specifications: foreign matter; loss on drying; total ash; and water extractable matter, using the methods prescribed by the WHO (1998) for quality control of medicinal plant materials. The trona was analyzed with methods adapted from BP (2004). The results suggest the existence of one variety of E. caryophyllata; but two varieties of S. bicolor and trona, differing in loss on drying; and two varieties of P. guineense and P. osun, differing in both total ash and water extractable matter. The implications of the results for good manufacturing practice are discussed.

Key words: Standardization, specifications, components, niprisan, phytomedicine, sickle cell disease.
 

International Journal of Medicinal Plants Research ISSN 2169-303X Vol. 14 (3), pp. 001-004, March, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Investigating Flavonoid-Induced Glycemic and Lipid Regulation from Nelumbo Nuficera Leaves in a Diabetic Mouse Model

Taoying Zhou1*, Denghong Luo1, Xingyuan Li1 and Yunbo Luo2

1Department of Biology, HuangGang Vocational and Technical College, HuangGang 438002, P. R. China.
2College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P. R. China.

Accepted 06 January, 2025

This study evaluated flavonoids extracted from lotus (Nelumbo nuficera Gaertn) on reducing hyperglycemia and hyperlipidemia in alloxan-induced diabetic mice. flavonoids from lotus(FLL) was orally administered once a day after 3 days of alloxan -induction at 50 and 200 mg/kg for 28 day, and the results showed that fasting blood glucose ,serum TC and TG levels were significantly decreased, whereas serum HDL-c level were increased. The dosage of 200 mg/kg is more effective than that of 50 mg/kg. In addition, FLL did not exhibit any toxic symptoms in the limited toxicity evaluation in male mice. The above results suggested that FLL could control blood glucose and modulate the metabolism of glucose and blood lipid. So we conclude that the FLL should be evaluated as a candidate for future studies on diabetes mellitus.

Key words: Hypoglycemic, hypolipidemic, flavonoids from lotus leaf.