ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Full length Research Paper
Investigation of Demodex spp. in perinea areas and faces of young men visiting the urology policlinic
Haluk Soylemez1, Ali Beytur2, Ilhan Gecit2, Ulku Karaman3*, Ali Ozer4 and Sinan Calik5
1Private Müjde Hospital, Urology Clinic, Malatya, Turkey.
2Beyda i State Hospital, Urology Clinic, Malatya, Turkey.
3Ordu University, School of Health, Ordu, Turkey.
4Department of Public Health, Sutcu mam University, Kahramanmaras, Turkey.
5Department of Statistics, Elazig Firat University, Turkey.
Accepted 23 July, 2017
Abstract
It has been reported that types of Demodex can be transmitted to other people through close contact and they can play a pathogenic role in rosacea, acne vulgaris, perioral dermatitis, seborrhoeic dermatitis, micropapillary-pruritic dermatitis, and blepharitis. Types of Demodex are reported to be located in various places of human body. The purpose of the present study was to detect the presence of Demodex spp. in the samples taken from the faces and perinea areas of the young men using standardized surface skin biopsy (SSSB). Accordingly, samples were obtained using SSSB method from the faces and perinea areas of 200 men aged 19 - 34, which were then covered with entellan and sent to the parasitological laboratory. Demodex spp. was found in 42.0% of the samples taken from perinea area and in 83.0% of the samples taken from subjects’ faces, while Enterobius vermicularis was detected in two cases. One of the samples taken from was Demodex brevis, while the others were Demodex folliculorum. It was concluded that, given that species of Demodex can be found in perinea area under conditions of collective accommodation, specimens should be taken from patients consulting with pruritus or allergic reactions in order to diagnose the parasite, and treatment protocol should be planned according to the results of the analysis.
Key words: Demodex spp., facies, perinea and cheek.
Ali Ozer and Sinan Calik, Ulku Karaman*, Ilhan Gecit, Haluk Soylemez, Ali Beytur
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Full Length Research Paper
Inhibition and control effects of the ethyl acetate extract of Trichoderma harzianum fermented broth against Botrytis cinerea
Guoliang Yin1, Weimin Wang1, Sha Sha1, Lei Liu2 and Xiaoping Yu1*
1College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
2Centre for Phytochemistry and Pharmacology, Southern Cross University, New South Wales 2480, Australia.
Accepted 18 June, 2017
Abstract
Botrytis cinerea is a widespread parasitic fungus that infects many crops and reduces their productivity. Trichoderma harzianum, also a fungus, has been commercially used as a biofungicide to control B. cinerea. The spores of T. harzianum, currently used in the application, have some drawbacks such as efficacy affected by the environmental condition and sensitivity to chemical fungicides. Instead of using the living microbe, the extract from T. harzianum fermented broth may be applied to control B. cinerea. It was found that the extract could inhibit mycelial growth (EC50 = 13.6 mg/L), conidia germination (EC50 = 17.5 mg/L) and conidia production (EC 50 = 23.6 mg/L) of B. cinerea. In vitro, the extract showed excellent control effects on tomato grey mould caused by B. cinerea and the protective effect (EC 50=99.6 mg/L) was better than the therapeutic effect (EC50=135.8 mg/L). In pot experiments, the extract also showed persistent protective (EC50=99.0 mg/L at day 7 and EC 50 = 142.7 mg/L at day 15) and therapeutic (EC 50 = 195.0 mg/L at day 7 and EC 50 = 393.7 mg/L at day 15) effects over 15 days. This study showed that the extract was equivalent or superior to the commercial dicarboximide fungicide, procymidone. In conclusion, the ethyl acetate extract of T. harzianum fermented broth is effective in the treatment of tomato grey mould caused by B. cinerea.
Key words: Botrytis cinerea, fungicide, tomato grey mould, trichodermin, Trichoderma harzianum.
Sha Sha, Lei Liu and Xiaoping Yu*, Guoliang Yin, Weimin Wang
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Full Length Research Paper
Isolation and characterization of Arthrobacter sp. HW08 capable of biodegrading swainsonine
Yan Wang, Yanchun Hu, Jianhua Wang*, Yongtao Yu, Yumin Song, Guodong Yang and Guoxia Geng
Department of Internal Medicine, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Shaanxi, China.
Accepted 21 July, 2017
Abstract
The aim of this study was to isolate bacteria capable of degrading swainsonine, an indolizidine alkaloid in poisonous plants of Oxytropis and Astragalus. The bacterium HW08 was isolated and enriched from soil and its physiological and biochemical properties, 16S rRNA gene and the ability of swainsonine-degradation were investigated. The results showed that HW08 was a species of Arthrobacter sp. Under the optimized temperature (30°C) and pH value (7.0), HW08 (OD600 = 0.3) could degrade about 2 mg swainsonine in 5 ml degrading reaction within 4 h. In conclusion, HW08 would be a better potential candidate to be considered for SW-degrading in practice.
Key words: Locoweeds, Arthrobacter; swainsonine, biodegradation.
Yan Wang, Yanchun Hu, Yumin Song, Guodong Yang and Guoxia Geng, Jianhua Wang*, Yongtao Yu
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Full Length Research Paper
Bacterial species associated with soils contaminated with used petroleum products in Keffi town, Nigeria
M. D. Makut* and P. Ishaya
Microbiology Unit, Department of Biological Sciences, Nasarawa State University, P. M. B. 1028, Keffi, Nigeria.
Accepted 25 July, 2017
Abstract
This investigation was carried out to determine the bacterial flora of soils contaminated with used oil (petroleum products) in Keffi town. Pour plate method involving the use of serial dilutions was employed for the isolation of the bacteria. Soil samples from 10 different mechanic workshops in Keffi town were obtained and plated out on plate count agar, eosin methylene blue agar, brilliant green agar, desoxycholate citrate agar and mannitol salt agar to isolate the bacterial species from the soils contaminated with petroleum products. The bacterial species isolated were Pseudomonas sp., Streptococcus sp., Escherichia coli, Staphylococcus sp., Klebsiella sp., Bacillus sp., Mycobacterium sp., Enterobacter aerogenes, Salmonella sp., and Micrococcus sp. The hydrocarbon substrates (petroleum products) used were petrol, kerosene, diesel and engine oil. Pseudomonas sp., Streptococcus sp., and Bacillus sp., were found to utilize all the four petroleum products as their sole source of carbon and energy. Staphylococcus sp., and Micrococcus sp., utilized petrol, kerosene and diesel, while Klebsiella sp., and Mycobacterium sp., utilized only petrol and diesel. Salmonella sp., E. aerogenes and E. coli did not utilize any of the test substrates (Petrol, kerosene, diesel and engine oil). The results of this study revealed that Pseudomonas sp., Streptococcus sp., and Bacillus sp., are the most versatile species of bacteria that could utilized petroleum products in the soil environment of Keffi. The investigation demonstrates that Pseudomonas sp., Streptococcus sp. and Bacillus sp. could be harnessed for use in bioremediation of land polluted with petroleum and petroleum products
Key words: Bacterial species, contaminated soils, used petroleum products, Keffi, Nigeria.
P. Ishaya, M. D. Makut*
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Full Length Research Paper
Morinda officinalis How enhances exercise endurance and possesses protective effects against oxidative stress of the rats after exercise
Zhang Dao-rong1*, Wan Yu1, Zu Jing1, Zhan Jian-guo2, Li Lei1 and Bai Jian-qing1
1College of physical education, Huaiyin Normal University, Huai'an, 223300, China.
2Beijing Sport University, Beijing, 100084, China.
Accepted 23 July, 2017
Abstract
The aim of this study was to investigate the effect of Morinda officinalis How (RMO) on exercise endurance capacity and exercise-related changes in lipid peroxidation. Thirty-two male Wistar rats were taken in the study, and the animals were divided into four groups (n = 8 per group) including one control group and three RMO administered groups (200, 400 and 800 mg/kg body weight). The RMO extracts were administered every day orally using intragastric tube for 30 days. Forced swimming test was performed with a weight corresponding to 3% body weight attached to the tail. Forced swimming time and biochemical parameters were measured. The result indicated RMO could enhance exercise endurance and possessed protective effects against oxidative stress of the rats after exercise. The study also revealed that RMO elevated superoxide dismutase (SOD) concentrations, suggesting that RMO were able to up-regulate antioxidant enzymes to protect against oxidative stress- induced injury after exercise. In addition, RMO also possessed the ability to retard and lower the blood lactate produced after exercise.
Key words: Morinda officinalis How, endurance capacity, lipid peroxidation, exercise.
Zhan Jian-guo, Zhang Dao-rong*, Wan Yu, Zu Jing, Li Lei and Bai Jian-qing
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Full Length Research Paper
Antimicrobial susceptibility of non-sorbitol fermenting Escherichia coli isolated from cattle feaces and milk samples
Joshua Orungo Onono*, Erastus Kiambi Kangethe and William Okelo Ogara
Department of Public Health, Pharmacology and Toxicology, University of Nairobi, P. O. Box 29053 - 00625, Nairobi, Kenya.
Accepted 15 June, 2017
Abstract
The objective was to determine the antimicrobial susceptibility of the non-sorbitol fermenting Escherichia coli colonies from cattle feaces and milk samples collected from Dagoretti division in Nairobi. A total of 285 feacal and 260 milk were collected from urban dairy farming households while non -dairy households provided 137 milk samples. The samples were used for culture and isolation of E. coli and the colonies isolated using standard microbiological methods. 23% (66) and 8.8% (23) of feacal and milk samples from urban dairy farming households had non sorbitol fermenting colonies, while 8.8% (12) of non-dairy farming household neighbours had non sorbitol fermenting colonies in milk samples. Antibiotic susceptibility patterns showed that isolates of E. coli were resistant to various antibiotics. There was a high percentage resistance to sulphamethoxazole in feacal samples isolates (14.4%), milk sample isolates (10%) from dairy farming household and milk sample isolates (11.7%) non-dairy households. The feacal isolates had a low resistance to ampicilin (1.4%), but the resistance in isolates from milk samples of urban dairy household (6.5%) and non-dairy household’s milk samples (7.3%) were high. The other antibiotics showed varied resistance pattern with feacal isolates having a high percentage resistance to tetracyclines (6.7%) while most bacterial isolates were susceptible to gentamicin. Multiple antibiotic resistances was observed in feacal sample isolates (6.7%), dairy farming household milk isolates (4.2%) and non- dairy farming household milk isolates (7.3%). Non-sorbitol fermenting E. coli colonies from cattle feaces and milk samples were resistant to most of the antibiotics tested and the higher percentage resistance to sulphamethoxazole, ampicilin and tetracyclines requires further investigation to isolate, identify and compare the genes responsible for development of resistance.
Key words: Non-sorbitol fermenting, Escherichia coli, urban dairy households, antimicrobial susceptibility.
Joshua Orungo Onono*, Erastus Kiambi Kangethe and William Okelo Ogara
Page: 1 - 10