ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-006, March, 2019. © International Scholars Journals
Full Length Research Paper
Enhanced cultivability of antagonistic bacterial strains from soft coral Sinularia sp., Gulf of Mannar, Southeastern India
C. Chellaram1*, T. Prem Anand2, D. Kesavan3, G. Priya4, M. Chandrika5 and C. Gladis5
1Department of Biomedical Engineering, Vel Tech Multi Tech Dr. RR SR Engineering College, Chennai – 600 062. TN. India.
2Vel Tech Dr. RR Dr. SR Technical University, Chennai-600062, TN. India.
3Department of Biotechnology, Vel Tech High Tech Dr. RR Dr. SR. Engineering College, Chennai, TN. India.
4Department of Industrial Biotechnology, Dr. M.G.R. University, Chennai. TN. India
5Department of Chemistry, Vel Tech Multi Tech Dr. RR SR Engineering College, Chennai – 600 062, TN. India.
Accepted 19 January, 2019
Abstract
Studies to increase the recoverability of antagonistic bacterial strains carried out from soft coral species Sinularia was cultured in a varied combination of low to high nutrient media. Each of the media was supplemented with sodium pyruvate. This study was aimed to find out whether the addition of sodium pyruvate to all solid media could significantly increase the recovery of microbes. Microorganisms on maltose amended seawater agar (2.1±0.1X104), followed by Free Lunch Medium (0.98±0.07X104) and OLIGO medium (1.61±0.4X104) which were highly recovered in the supplemented media than in non-supplemented media. These findings suggest that the addition of sodium pyruvate to solid growth and isolation media may improve recoverability of microorganisms from soft coral. The results also indicated that the higher percentage of the antagonistic bacteria was found on supplemented media when compared to that of the non-supplemented media. Hence, the highest percentage of antagonistic bacteria was found to be exhibited by the supplemented media of MA+SE media, which are isolated from Sinularia sp. was (24.89%).
Key words: Improved recoverability, antagonistic, sodium pyruvate, Sinularia sp.
D. Kesavan, M. Chandrika and C. Gladis5, C. Chellaram*, T. Prem Anand, G. Priya
Research Article
Serum paraoxonase and arylesterase activities and oxidant status in patients with brucellosis
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-006, March, 2019. © International Scholars Journals
Full Length Research Paper
Serum paraoxonase and arylesterase activities and oxidant status in patients with brucellosis
Hasan Karsen1*, Mustafa Kasım Karahocagil2, Hayrettin Akdeniz2, Mehmet Re at Ceylan3, Irfan Binici3, Sahbettin Selek4 and Hakim Celik4
1Department of Infectious Diseases and Clinical Microbiology, Harran Univesity School of Medicine, Sanliurfa, Turkey.
2Department of Infectious Diseases and Clinical Microbiology, Yuzuncu Yil Univesity School of Medicine, Sanliurfa, Turkey.
3Department of Infectious Diseases and Clinical Microbiology, Yuzuncu Yil Univesity School of Medicine, Van, Turkey.
4Department of Biochemistry, Harran Univesity School of Medicine, Sanliurfa, Turkey.
Accepted 23 January, 2019
Abstract
The aim of this study was to the investigate the effect of brucellosis infection on paraoxonase-1 (PON1) activity and oxidative status in patients with brucellosis. Sixty patients with brucellosis (47 acute, 13 subacute) and the 67 healthy controls were used in the study. Serum PON1 and arylesterase activities, total antioxidant capacity (TAC), total oxidant status (TOS), oxidative stress index (OSI), total free sulfhydryl (-SH), and lipid hydroperoxide (LOOH) groups were determined. In addition, HDL-cholesterol (HDL- C), LDL-cholesterol (LDL-C), total cholesterol (TC) and triglyceride (TG) were measured. Serum PON1 and arylesterase activities, total - SH group levels and TAC were significantly lower in brucellosis patients than controls (P<0.001). TOS, OSI and LOOH levels were significantly higher in brucellosis patients than controls (P<0.001). There were no significant differences between of serum LDL-C, TG and TC levels of patients subjects compared to controls (P > 0.05). Patients with brucellosis are exposed to potent oxidative stress and they have decreased PON1 activity. These predisposal factors might play a role in the pathogenesis for atherosclerosis in patients with brucellosis.
Key words: Brucellosis, PON1 activity, oxidative status, atherosclerosis.
Hayrettin Akdeniz, Mustafa Kasım Karahocagil, Sahbettin Selek and Hakim Celik, Hasan Karsen*, Irfan Binici, Mehmet Re at Ceylan
Research Article
Outer membrane proteins and morphological alterations of Shigella spp. under starvation in seawater
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-006, February, 2019. © International Scholars Journals
Full Length Research Paper
Outer membrane proteins and morphological alterations of Shigella spp. under starvation in seawater
Ali Ellafi*, Fethi Ben Abdallah, Rihab Lagha and Amina Bakhrouf
Faculty of Pharmacy, University of Monastir, Avenue Avicenne, Monastir 5019, Tunisia.
Accepted 04 January, 2019
Abstract
In this study, we incubated four strains of Shigella in seawater microcosms (at room temperature and at 4°C) for eight months and we studied the alteration of their morphologic and outer membranes proteins. The starved cells showed an evolution to the filterable minicells state capable to pass membrane pore size 0.45 µm. In addition, the atomic force micrographs showed a reduction of the cells size and an evolution to coccoid-shapes. Outer membrane proteins patterns of stressed bacteria did not changed too much and these modifications were manifested by the appearance of one new band.
Key words: Shigella, seawater, starvation, morphological, outer membrane proteins.
Rihab Lagha and Amina Bakhrouf, Fethi Ben Abdallah, Ali Ellafi*
Review
An overview of honey: Therapeutic properties and contribution in nutrition and human health
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-009, February, 2019. © International Scholars Journals
Review
An overview of honey: Therapeutic properties and contribution in nutrition and human health
Christy E. Manyi-Loh1, Anna M. Clarke1 and Roland N. Ndip1,2*
1Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Fort Hare, Microbial Pathogenicity and Molecular Epidemiology Research Group, P/Bag X1314, Alice 5700, South Africa.
2Department of Biochemistry and Microbiology, Faculty of Science, University of Buea, Box 63, Buea, Cameroon.
Accepted 16 January, 2019
Abstract
Honey, a natural product of very high nutritive value is made when the nectar (floral) and sweet deposits from plants (non floral) are gathered, modified and stored in the honeycombs by honeybees of the genera Apis and Meliponini. Its composition and quality vary greatly with the botanical source of nectar as well as environmental and climatic conditions. Depending on its quality, honey can contribute to the health and nutritional status of humans. These beneficial actions have been ascribed to its antimicrobial, anti-inflammatory and anti-oxidant potential. Interestingly, honey is gradually receiving attention as a complementary and or an alternative source of treatment in modern medicines. It is active against antibiotic-sensitive and antibiotic-resistant strains of micro-organisms and has the potential not to select for further resistant strains.
Key words: Honey, natural product, drug, food.
Anna M. Clarke and Roland N. Ndip*, Christy E. Manyi-Loh
Research Article
Status of Citrus tristeza virus (CTV) in Peninsular Malaysia
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-006, February, 2019. © International Scholars Journals
Full Length Research Paper
Status of Citrus tristeza virus (CTV) in Peninsular Malaysia
Kavous Ayazpour1, Kamaruzaman Sijam1*, Ganesan Vadamalai1, Hawa Jaafar2
1Plant Protection Department, Faculty of Agriculture, University Putra Malaysia, 43400, Malaysia.
2Crop Science Department, Faculty of Agriculture, University Putra Malaysia, 43400, Malaysia.
Accepted 08 January, 2019
Abstract
Citrus tristeza virus (CTV) is the most important viral disease of Citrus spp. and has a worldwide distribution. Results of ELISA and PCR showed that all Citrus varieties including Fortunella sp., Citrofortunella microcarpa and Citromelo in major citrus growing areas of Malaysia had a high infection rate with CTV. In most areas, pomelo however was free of infection, but in Cameron Highlands, we found some strains of CTV that were severe to Citromelo and pomelo. Phylogeny studies revealed that these strains were similar to CTV isolates from China and Japan and were very different from CTV isolates from USA and New Zealand.
Key words: Citrus tristeza virus, phylogeny analysis, strain, CP gene, Malaysia.
Ganesan Vadamalai, Kavous Ayazpour, Hawa Jaafar, Kamaruzaman Sijam*
Research Article
Screening of higher yield of ethyl acetate yeast and researching of ester-producing conditions
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-006, February, 2019. © International Scholars Journals
Full Length Research Paper
Screening of higher yield of ethyl acetate yeast and researching of ester-producing conditions
MaoBin Chen1, Ruili Li1, Nan Peng2, Da Zhen1 and Shangling Fang1*
1Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China.
2State key laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R. China.
Accepted 19 January, 2019
Abstract
A higher yield of ethyl acetate yeast Y1 was selected from Daqu and Xiaoqu, and the production volume of ethyl acetate was 2.152 g/l when cultured in corn saccharification mash. The strain is identified as Hansenula anomala. Then, through the single factor test to study the effects of diffident culture mediums and diffident culture methods on ester-producing, the results showed that the best ester-producing culture medium for Y1 yeast was the wheat saccharification mash. The ester yield of static culture was 40% higher when compared with shaking culture. several factors were selected to carry on the orthogonal test once more, the result indicated that the optimization plan were as follows: brix was 8°, pH was 5, temperature was 25°C, culture time was 4 days, and ethyl alcohol was 2%, total ester production of Y1 was 4.812 g/l in this condition.
Key words: Ester–production yeast, culture method, ester production condition, orthogonal test.
Ruili Li, MaoBin Chen, Da Zhen and Shangling Fang*, Nan Peng