ISSN 2736-1756
Research Article
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full Length Research Paper
Monogenean parasites in Sparid fish (Pagellus genus) in eastern Algeria coastline
Nouha Kaouachi1*, Chahinez Boualleg1, Mourad Bensouilah1 and Bernard Marchand2
1Laboratoire d’Ecobiologie des Milieux Marins et Littoraux, Faculté des Sciences, Université Badji Mokhtar Annaba, BP: 12 Annaba, 23000, Algérie.
2Université de Corse, CNRS UMR 6134, Faculté des Sciences et Techniques, Laboratoire (Parasites et écosystèmes méditerranéens), BP 52, 20250 Corte, France.
Accepted 22 April, 2015
Abstract
A spatial distribution study of parasite populations of gills from 540 specimens belonging to Pagellus genus: Pagellus acarne, Pagellus erythrinus and Pagellus bogaraveo (Sparidae) was realized from the eastern Algeria coastline. A preliminary description allows the distinguishing of eight parasite species belonging to two subclasses: Monopisthocotylea and Polyopisthocotylea. Rates of parasite species and parasitic indices of host species were calculated. The study findings show that the parasitic populations vary depending on the site and the host species within the same genus.
Key words: Parasitism, monogenean, Pagellus erythrinus, Pagellus acarne, Pagellus bogaraveo, Algerian coastline.
Chahinez Boualleg, Nouha Kaouachi*, Mourad Bensouilah and Bernard Marchand
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full Length Research Paper
Occurrence of tetracycline-resistant Aeromonas hydrophila infection in Korean cyprinid loach (Misgurnus anguillicaudatus)
Jin Woo Jun, Ji Hyung Kim, Dennis K. Gomez, Casiano H. Choresca Jr, Jee Eun Han, Sang Phil Shin and Se Chang Park*
Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
Accepted 12 April, 2016
Abstract
In this paper, we described a mass mortality of cyprinid loaches, Misgurnus anguillicaudatus from a private fish farm in Korea. Diffuse bleeding was observed on the skin around the anal orifice. Bacterial pathogens from kidneys samples of moribund fish were cultured, identified and confirmed to be Aeromonas hydrophila using morphological, biochemical test and genetical analysis. The isolated A. hydrophila strains were resistant to commercial antibiotics and the presence of the tetracycline resistance gene (tet E) was detected by genetical analysis. Pathogenecity test was performed using healthy 4-week-old cyprinid loaches by intraperitoneal (IP) injection and the LD50 concentration of the pathogen was determined to be 6.0 × 107 CFU fish-1. In this paper, we confirmed the infection of farm-raised cyprinid loaches with tetracycline-resistant A. hydrophila.
Key words: Cyprinid loach (Misgurnus anguillicaudatus), Aeromonas hydrophila, tetracycline resistance gene, pathogenecity test.
Ji Hyung Kim, Jee Eun Han, Casiano H. Choresca Jr, Jin Woo Jun, Sang Phil Shin and Se Chang Park*, Dennis K. Gomez
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full Length Research Paper
Bacteria population of some commercially prepared yoghurt sold in Enugu State, Eastern Nigeria
Nwagu Tochukwu Nwamaka1* and Amadi Emmanuel Chike2
1Department of Microbiology and Biotechnology, University of Nigeria, Nsukka, Enugu State, Nigeria.
2Department of Microbiology and Biotechnology, Caritas University Amorji Nike, Enugu State, Nigeria
Accepted 18 March, 2015
Abstract
The demand for fermented dairies is on the increase in developing countries following consumer awareness of some of the health benefits attributed to prolonged intake. Eight samples of yoghurt produced from Enugu, Eastern Nigeria was collected from various locations. Samples were incubated using various media for the isolation, identification and enumeration of the bacteria population within the yoghurt. Reports indicated that total viable count of bacteria was in the range of 1.4 x 106 – 2.2 x 107 cfu /ml. Not all bacterial isolates were lactose fermenters. Members of the genus Staphylococcus, Aeromonas, Klebsiella, Pseudomonas, Bacillus, Streptococcus and Lactobacillus were isolated with 100, 25, 50, 33.3, 25, 12.5 and 25% frequency of occurrence, respectively. Viable counts of lactic acid bacteria were low indicating that probiotics effect following consumption may be poor. Occurrence of pathogenic organisms indicates improper handling and inadequate sanitary measures. More care should be taken during yoghurt fermentation.
Key words: Lactobacillus, fermentation, yoghurt, bacteria, contaminant.
Nwagu Tochukwu Nwamaka*, Amadi Emmanuel Chike
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full length Research Paper
Influences of light and temperature on membrane potential and respiratory viability of an aerobic anoxygenic phototrophic bacterium Erythrobacter sp. JL475
Zaiqing Peng and Nianzhi Jiao*
State Key Laboratory for Marine Environmental Science, Xiamen University, Xiamen, 361005, P. R. China.
Accepted 6 May, 2015
Abstract
Flow cytometry’s (FCM) measurement of membrane potential (MP) and cell respiration viability based on continuous culture was used to investigate the responses of aerobic anoxygenic phototrophic bacteria (AAPB) in the heterotrophic growth and regulation mechanism of photosynthesis to environmental changes. An AAPB strain Erythrobacter sp. JL475 and a non-AAPB strain Erythrobacter sp. JL316 were used as the experimental bacteria, both of which were isolated from the South China Sea. The results showed that light -cultured AAPB showed higher MP and biomass at 10°C, suggesting an obvious stimulation of light on AAPB growth. By contrast, dark-cultivated JL475 had higher MP and biomass at higher temperature (20, 30 and 40°C). The rate of heterotrophic respiration at different temperature environment ranked as follows: dark-cultivated JL316 > dark-cultivated JL475 > light/dark cycling cultivated JL475. Light undoubtedly increased the cell viability of AAPB, especially of apoptosis cells. The CTC+% at different carbon concentration ranked as follows: light/dark cycling cultivated JL475 > dark-cultivated JL316 > dark-cultivated JL475. It was concluded that the heterotrophic respiration would played a key role in energy metabolism of AAPB, photosynthesis may provide an advantage for AAPB to survive in a variety of diverse environments.
Key words: Flow cytometry, aerobic anoxygenic phototrophic bacteria, membrane potential, respiration viability, biomass.
Nianzhi Jiao*, Zaiqing Peng
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full Length Research Paper
Isolation, recombinant expression and characterization of the dprA gene product in Streptomyces rimosus NRRL 2455
Khaled Mohamed Anwar Aboshanab
Microbiology and Immunology department, Faculty of Pharmacy, Ain Shams University, Organization of African Unity St., POB: 11566, Abbassia, Cairo, Egypt. E-mail: [email protected]. Tel. 002-0107582620.
Accepted 22 March, 2015
Abstract
Dihydrodipicolinate synthase (DHDPS) catalyses the first enzymatic step in the lysine-biosynthetic pathway in bacteria, plants and some fungi. In this study, cloning and submission of 3639 bp from Streptomyces rimosus NRRL 2455 to the EMBL database under the accession code EU617017 were carried out. The entire dprA gene was cloned and heterologously expressed in Escherichia coli BL21 (DE3) and the gene product was purified and analysed spectrophotometrically. A dprA- knock-out mutant was created and showed a reduction of mycelia growth and spore formation by about 43 and 37%, respectively, as compared to the wild strain. Fed with meso-diaminopimelic acid, the dprA- mutant regained its capability of mycelial growth, however no significant effect on spore formation was observed confirming that dprA gene product was involved in the biosynthesis of dihydrodipicolinate in S. rimosus NRRL 2455. This is the first report about identification of dihydrodipicolinate synthase of the order streptomycetes.
Key words: Dihydrodipicolinate synthase, lysine biosynthesis, Streptomyces rimosus NRRL 2455, dprA knock-out mutants
Khaled Mohamed Anwar Aboshanab
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full Length Research Paper
Research advance and superiority of microdiesel production with biowastes
Chuan-chao Dai*, Jie Tao, Yu Wang and Yu-min Hao
Jiangsu Key Laboratory for Biodiversity and Biotechnology , College of Life Science, Nanjing Normal University, Nanjing 210046, People’s Republic of China.
Accepted 27 January, 2015
Abstract
Lignocellulosic biowastes can be utilized to produce microdiesel, a promising alternative energy source for limited crude oil and food based biofuels. As an alternative fuel, microdiesel produced from biowastes it has superior environmental benefits, economically competitive, producible in sufficient quantities and the energy conversion efficiency is also estimated in the article in theory. Microdiesel derived from biowastes is a potential renewable and carbon neutral alternative to petroleum fuels. Unfortunately, microdiesel from biowastes cannot realistically satisfy even a small fraction of the existing demand for transport fuels. As demonstrated here, microdiesel appears to be the only source of renewable biodiesel that is capable of meeting the global demand for transport fuels. Approaches for making microdiesel economically competitive with petrodiesel are also discussed.
Key words: Microdiesel, lignocellulosic biowastes, environmental benefits, potential supply.
Yu Wang and Yu-min Hao, Jie Tao, Chuan-chao Dai*
Page: 1 - 10