ISSN 2736-1756
Short Communication
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Short Communication
Frequency and anti-biogram pattern of coagulase negative Staphylococcus in clinical specimens of Shahid Mohammadi Hospital in patients, Bandar-Abbas, Iran
Sedigheh Javadpour1,3*, Ehsan Karimi1 and Afsaneh Karmostaji2
1Department of Microbiology, Infectious Disease Research Center of Bandar Abbas, Iran.
2Premedical Faculty of Bandar_Abbas, Hormozgan University of Medical Sciences, Iran.
3Department of Microbiology, Bandar_Abbas Faculty of Medicine, Shahid Nasser Ave, Bandar Abbas, Iran.
Acceptance 7 June, 2017
Abstract
Coagulase negative staphylococci (CoNS), commonly known as a part of normal-biota of human body, have become predominant pathogens over the last decades. Due to the vast use of antibiotics for therapy or prophylaxis, those CoNS strains acquired in the hospitals have become resistant to various antimicrobial agents. This descriptive cross-sectional study was carried out from December 2007 to June 2008 on 5063 clinical samples. Identification of CoNS was performed by routine microbiological methods using gram staining, catalase and coagulase tests. Susceptibility of microorganisms to 14 antibiotics was determined by Disc Diffusion Method. Among 1573 positive cultures, 17.5% of isolates were CoNS. About 74% of isolates belonged to out patients and 26% of them were obtained from hospitalized patients. Most of CONs were isolated from internal (38.9%) and internal emergency wards (34.7%). Among different clinical samples, CoNS were mainly isolated from urine cultures (78.6%) in women. Vancomycin (100%) and Imipenem (94%) were found to be the most effective antibiotics. Ciprofloxacin, norfloxacin and aminoglycosides were effective antibiotics to combat CoNS in out patient department (OPD patients).
Key words: Coagulase negative staphylococci, inpatients, antibiogram pattern.
Sedigheh Javadpour*, Ehsan Karimi and Afsaneh Karmostaji
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Full Length Research Paper
Isolation, identification and characterization of multiple enzyme producing actinobacteria from sediment samples of Kodiyakarai coast, the Bay of Bengal
P. Manivasagan*, S. Gnanam, K. Sivakumar, T. Thangaradjou, S. Vijayalakshmi and T. Balasubramanian
CAS in Marine Biology, Annamalai University, India.
Accepted 25 February, 2017
Abstract
A total of twenty nine strains were isolated from the Kodiyakarai coastal sediments (Bay of Bengal) and ten of them exhibited multiple enzyme activity. Of these, the best (GK-22) was selected based on zone formation (amylase, cellulase and protease) and its growth conditions were standardized for better production of multiple enzymes. Present study on production of multiple enzymes (amylase, cellulase and protease) by GK-22 showed higher enzyme levels at the end of the stationary phase after incubation for 72 h at pH 7.0. Maximum activity of amylase, cellulase and protease (84, 88 and 89 IU/ml, respectively) was obtained at pH 7.0, temperature 45°C, sodium chloride concentration 2%, carbon compound sucrose, nitrogen compound beef extract, amino acid L-asparagine for amylase and cellulase and L-tyrosine for protease. The multiple enzymes were purified by precipitation with ammonium sulphate and ion exchange chromatography and the SDS-PAGE showed a single band for the purified enzyme, with an apparent molecular weight of 80 (amylase), 66 (cellulase) and 97 KDa (protease). The strain, GK-22 which showed higher multiple enzyme activity was tentatively identified as Streptomyces alboniger. These findings suggest that the strain can effectively be used in large scale production of multiple enzymes for commercial purposes, after testing and ascertaining the strain’s capability in large scale fermentations.
Key words: Amylase, cellulase, protease, actinobacteria, Kodiyakarai, Streptomycetes alboniger.
P. Manivasagan*, S. Vijayalakshmi and T. Balasubramanian, T. Thangaradjou, K. Sivakumar, S. Gnanam
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Full Length Research Paper
Comparison of the effect of particulate materials and some osmoregulators on lactic fermentation of new local white cassava variety “Bianbasse” using both spontaneous and starter cultures
L. A. Adetunde1* and A. A. Onilude2
1Department of Botany and Microbiology, Faculty of Applied Sciences, University for Development Studies, Navrongo Campus, Navrongo, UER, Ghana.
2Department of Botany and Microbiology, University of Ibadan, Ibadan, Oyo State, Nigeria.
Accepted 22 June, 2016
Abstract
The effects of particulate materials and osmoregulatators on lactic fermentation of cassava were determined on total dissolved loads of all the samples; the total reducing sugars of all samples, the microbial loads in all the samples, the percentage crude protein contents, crude fibres, crude fat/ether, ash, phytic acid and tannin. Sample A1 inoculated with varied concentrations of particulate materials had the highest total dissolved solid, total reducing sugar, lactic acid bacteria counts and total bacteria counts than sample B1 with varied concentrations of osmoregulator. There was corresponding increase in sample A2 and A3 compared to sample B2 and B3. Sample C which served as control had the lowest value in all at 24, 48 and 72 h of fermentation. Most of the samples that contained varied concentration particulate materials had higher values in their proximate analysis and nutritional analysis than samples that contained varied concentrations of osmoregulators. Sample C with neither particulate materials nor osmoregulator had the least values in all analysis.
Key words: Particulate materials, osmoregulatators, lactic fermentation, samples.
A. A. Onilude, L. A. Adetunde*
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Full Length Research Paper
Bacteria associated with bovine dermatophilosis in Zaria, Nigeria
J. S. Dalis1*, H. M. Kazeem2, A. A. Makinde1 and M. Y. Fatihu2
1National Veterinary Research Institute, Vom, Nigeria.
2Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria.
Accepted 18 March, 2017
Abstract
A study was carried out to determine the type of bacteria associated with bovine dermatophilosis in Zaria, Nigeria. Skin samples obtained from two hundred and eleven cattle with skin lesions suspected to be dermatophilosis were processed for bacteriology. One hundred and sixty-seven (79.1%) samples were positive for Dermatophilus congolensis, while 44 (20.9%) were negative. Both D. congolensis-positive and D. congolensis-negative samples were processed for isolation of other bacteria and the data was analyzed using Chi square test. Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, Micrococcus spp, Corynebacterium spp., Escherichia coli, Proteus spp and Pseudomonas spp . were isolated from both D. congolensis-positive and D. congolensis-negative scabs. However, the rate of recovery of S. aureus from D. congolensis-positive cattle was significantly (P < 0.05) higher than the rate of its recovery from D. congolensis negative cattle. There was no significant difference (P > 0.05) between the occurrence of the other isolates in D. congolensis- positive and D.congolensis-negative cattle. It was concluded that S. aureus could be a major complicating factor in naturally occurring dermatophilosis of cattle. The need to investigate the role of bacteria particularly that of S. aureus in the development of bovine dermatophilosis was emphasized.
Key words: Dermatophilus congololensis, bovine skin, associated bacteria, Zaria, Nigeria.
A. A. Makinde and M. Y. Fatihu, J. S. Dalis*, H. M. Kazeem
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Full length Research Paper
Infection pathways of soft rot pathogens on Amorphophallus konjac
Jinping Wu1,2, Ying Diao1, Yucheng Gu2 and Zhongli Hu1*
1Key Lab of the Ministry of Education for Plant Developmental Biology, College of Life Science, Wuhan University, Wuhan 430072, China.
2Industrial Crops Institute of Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Accepted 20 May, 2017
Abstract
With the rapid expansion of cultivated land, bacterial soft rot of konjac is more and more serious. The main pathogen is Erwinia carotovora subsp. carotovora. Some researchers think the natural openings and wounds are the main infection pathways on konjac. In this study, the infection pathways are investigated. From macro-observation, the soft-rot pathogens could not directly invade the intact corms, but it could invade the infected corms through the new rots. At the same time, bud scales, roots and wounded corms could be directly infected. From micro-observation, the epidermis of the corm had no stomas and lenticels, the stomas of the bud scales had no pathogens gathered around, and the pathogens broke down the infected roots and bud scales cells layer by layer. The results showed that soft rot pathogens invaded Amorphophallus konjac through the wounds and the growth of the organizations rather than the natural openings.
Key words: Amorphophallus konjac, bacterial soft rot, infection pathways.
Yucheng Gu and Zhongli Hu*, Ying Diao, Jinping Wu
Page: 1 - 10
Research Article
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Full length Research Paper
Plant growth promoting activities of fluorescent pseudomonads associated with some crop plants
A. Suresh, P. Pallavi, P. Srinivas, V. Praveen Kumar, S. Jeevan Chandra and S. Ram Reddy*
Department of Microbiology, Kakatiya University, Warangal- 506009 (A.P), India.
Accepted 13 March, 2017
Abstract
Fluorescent pseudomonads (FP), a major component of rhizobacteria, promote the plant growth through their multifarious activities. In the present investigations, 10 strains of fluorescent pseudomonads isolated from the rhizosphere soils of bajra (Pennisetum glaucum), jowar (Sorghum vulgare), rice (Oryza sativa) and maize (Zea mays) were screened for their plant growth promoting activity based on their ability to produce hydrogen cyanide (HCN), siderophores, proteases, indole acetic acid (IAA), broad spectrum antifungal activity against pathogenic fungi and phosphate solubilization. The results indicated that most of the isolates tested possess plant growth promoting traits. These isolates can be used as potential biofertilizers and also as biocontrol agents.
Key words: PGPR, indole acetic acid, hydrogen cyanide, protease, phosphate solubilization, antagonism.
P. Pallavi, S. Jeevan Chandra and S. Ram Reddy*, V. Praveen Kumar, A. Suresh, P. Srinivas
Page: 1 - 10