ISSN 2326-7267
Research Article
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (11), pp. 001-006, November, 2017. © International Scholars Journals
Full Length Research Paper
Clinical evaluation of green tea polyphenols in treatment of pharyngitis in human subjects
Y. F. Wang1, L. Ying1, P. Xu1, B. Y. Zhang2, X. Q. Yang1 and L. S. Qian1*
1Department of Tea Science, Zhejiang University, Hangzhou, 310029, P.R. China.
2Tiantai People’s Hospital, Tiantai, 317200, P.R. China.
Accepted 25 March, 2017
Abstract
Tea from Camellia sinensis (L.) Kuntze has long been used in herb medicine and recognized to possess anti-inflammative effects. The aim of the current study was to evaluate the therapeutic effects of green tea polyphenols in treatment of pharyngitis in human subjects. The clinical study was carried out as a randomized, double- blind, parallel- group comparative trial. It was found that the integral scores of all the observed individual clinical signs and symptoms were decreased significantly from pre-treatment to post-treatment (P<0.05) in both green tea polyphenols treatment and the control. Therapeutic effects assessment showed that the decrease in the total integral scores in the green tea polyphenols group was more significant (P<0.05) than in the control medicine group and that green tea polyphenols had a more prominent efficacy (P<0.05) in eliminating pharyngodynia in pharyngitis patients. Green tea polyphenols demonstrated high total effective rate in the pharyngitis treatment (n = 60, 96.67%). Neither adverse events nor abnormal observation in vital signs were found throughout the study. It can be concluded that green tea polyphenols can be used as a reliable and effective phytochemical in the treatment of pharyngitis.
Key words: Green tea polyphenols, clinical evaluation, pharyngitis, human subjects.
X. Q. Yang and L. S. Qian*, L. Ying, Y. F. Wang, P. Xu, B. Y. Zhang
Page: 1 - 6
Research Article
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (11), pp. 001-009, November, 2017. © International Scholars Journals
Full Length Research Paper
Spica Prunellae extract promotes mitochondrion-dependent apoptosis in human colon carcinoma cell line
Liangpu Zheng1, Youqin Chen2,4, Wei Lin1, Qunchuan Zhuang1, Xuzheng Chen1, Wei Xu3, Xianxiang Liu1, Jun Peng1* and Thomas J. Sferra2,4
1Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, 1 Huatuo Road, Minhou Shangjie, Fuzhou, Fujian 350108, China.
2Department of Pediatrics, The University of Oklahoma Health Sciences Center, 940 Stanton L. Young Boulevard, Oklahoma City, OK 73190, USA.
3Department of Pharmacology, Fujian University of Traditional Chinese Medicine, 1 Huatuo Road, Minhou Shangjie, Fuzhou, Fujian 350108, China.
4Department of Biochemistry and Molecular Biology, The University of Oklahoma Health Sciences Center, 940 Stanton L. Young Boulevard, Oklahoma City, OK 73190, USA.
Accepted 14 April, 2017
Abstract
Spica Prunellae (Prunella vulgaris fruiting spikes) has long been used as an important component in formulated prescriptions of Chinese traditional medicine to treat various kinds of cancer. However, the precise mechanism of the anti-cancer activity of Spica Prunellae remains to be elucidated. In this report, we investigated the cellular effects of the ethanol extract of Spica Prunellae (EESP) in the HT-29 human colon carcinoma cell line. We found that EESP inhibited the growth of HT-29 cells as evidenced by EESP-induced cell morphological changes and reduced cell viability in dose- and time-dependent manners. Furthermore, we demonstrated that the HT-29 cell growth inhibitory activity of EESP was due to apoptosis, as EESP treatment resulted in the loss of plasma membrane asymmetry (externalization of phosphatidylserine), collapse of mitochondrial membrane potential, activation of caspase-9 and caspase-3, and increase in the ratio of pro-apoptotic Bax to anti-apoptotic Bcl-2. Taken together, these results suggest that Spica Prunellae inhibits the growth of HT-29 colon cancer cells through mitochondrion-mediated apoptosis, which may, in part, explain its anti-cancer activity.
Key words: Apoptosis, anti-tumor, HT-29 cells, phytotherapy, mitochondria, Spica Prunellae.
Qunchuan Zhuang, Youqin Chen, Xuzheng Chen, Liangpu Zheng, Wei Xu, Xianxiang Liu, Wei Lin, Jun Peng* and Thomas J. Sferra
Page: 1 - 9
Research Article
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (10), pp. 001-005, October, 2017. © International Scholars Journals
Full Length Research Paper
Cytotoxic evaluation of fluvastatin and rosuvastatin and effect of fluvastatin in the hela cell cycle
María Campos-Lara1 and José Alberto Mendoza-Espinoza2*
1Unidad de Investigación Médica en Farmacología, Centro Médico Nacional Siglo XXI, México City, México.
2Laboratorio de Productos Naturales, Plantel Casa Libertad, Universidad Autónoma de la Ciudad México, Calzada Ermita Iztapalapa Num. 4163, Col. Loma de Zaragoza, 09620 C. P., Delegación Iztapalapa, México City, México D.F., México.
Accepted 13 July, 2017
Abstract
3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, were originally designed to reduce cholesterol biosynthesis and have been extensively used as prevention drugs against hyperlipidemia and cardiovascular conditions. Recently, these compounds have been shown to display chemopreventive activity against cancer. However, the effects of statins on cancer are not completely understood. For this reason, we have studied the cytotoxic effect of rosuvastatin and fluvastatin on three cell tumoral lines: human larynx carcinoma (HEp- 2), human nasopharyngeal carcinoma (KB), and human epithelial carcinoma (HeLa). We have found that only fluvastatin has relevant activity against the tumoral cell lines assayed and the capacity to arrest G1- phase, whereas a significant decline was observed in the S-phase percentage. Fluvastatin IC50 were 2.43±0.56 mg/mL (HEp-2), 2.29±0.19 mg/mL (KB), and 5.02±1.52 mg/mL (HeLa), whereas rosuvastatin showed poor activity. These results indicate that the cytotoxic effect of fluvastatin may not depend directly on HMG-CoA reductase inhibition. The antitumor statins effect needs further investigation.
Key words: Cell cycle, cytotoxicity, statins.
José Alberto Mendoza-Espinoza*, MarÃa Campos-Lara
Page: 1 - 5
Research Article
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (10), pp. 001-005, October, 2017. © International Scholars Journals
Full Length Research Paper
Immunomodulatory activity of various extracts of Adhatoda vasica Linn. in experimental rats
G. Vinothapooshan1* and K. Sundar2
1Arulmigu Kalasalingam College of Pharmacy, Krishnankoil – 626 190, Tamil Nadu, India.
2Department of Biotechnology, Kalasalingam University, Krishnankoil – 626 190, Tamil Nadu, India.
Accepted 15 April, 2017
Abstract
Methanolic, chloroform and diethyl ether extracts of leaves of Indian medicinal plant Adhatoda vasica Linn . were pharmacologically validated for its immunomodulatory properties in experimental animals. Oral administration of extracts at a dose of 400 mg/kg in adult male Wister rats significantly increased the percentage neutrophil adhesion to nylon fibers (P<0.001). It extracts were also found to induce Delayed Type Hypersensitivity reaction by sheep erythrocytes (P< 0.001). The observed results at different doses were significant when compared to control groups. These findings suggested that the extracts of this plant, A. vasica Linn positively modulates the immunity of the host.
Key words: Adhatoda vasica Linn., immunomodulatory, neutrophil.
G. Vinothapooshan*, K. Sundar
Page: 1 - 5
Research Article
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (9), pp. 001-006, September, 2017. © International Scholars Journals
Full Length Research Paper
Sex based pharmacological treatment in patients with metabolic syndrome: Findings from the Isfahan healthy heart program
Mojgan Gharipour1*, Roya Kelishadi2, Nafiseh Toghianifar2, Mahsa Mackie3, Mehrdad Yazdani2, Fatemeh Noori4 and Nizal Sarrafzadegan2
1Department of Biochemistry, Isfahan Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
2Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
3Centre for Neuroscience, University of Alberta, Canada.
4Statistical sciences, Isfahan Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
Accepted 18 March, 2017
Abstract
Pharmacological therapy is a critical step in the management of individuals with the metabolic syndrome (MetS) when lifestyle modifications cannot achieve the therapeutic goals. However, it has been well- documented that there is no single best therapy other than weight loss, and that treatment should be targeted at individual components of the MetS. The objective of this study was to investigate the treatment of individual components of the MetS in a population-based sample of individuals with clustering MetS components. In a cross-sectional population-based survey, we studied a randomly collected sample of men and women who had participated in the baseline survey of a community-based program in three Central Iranian counties in 2000 to 2001. Demographic data, medical status, and drug history were obtained by questionnaire. We evaluated the association between clustering of the MetS components and pharmacological treatment of cardiovascular risk factors. The most common pharmacologic agents prescribed for individuals with the MetS were beta-blockers (72.8%), followed by lipid-lowering agents (36%) with no significant gender difference. A high level of compliance with drug treatment was noted. Further research is warranted to understand the compliance behavior of patients with the MetS.
Key words: Iran, metabolic syndrome, pharmacological treatment, compliance.
Mehrdad Yazdani, Mojgan Gharipour*, Roya Kelishadi, Nafiseh Toghianifar, Mahsa Mackie, Fatemeh Noori and Nizal Sarrafzadegan
Page: 1 - 6
Research Article
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (9), pp. 001-005, September, 2017. © International Scholars Journals
Full Length Research Paper
Element content analysis of plants of genus Ficus using atomic absorption spectrometer
Kiran Yasmin Khan1*, Mir Ajab Khan1, Rabia Niamat1, Mamoona Munir1, Hina Fazal2, Paras Mazari1, Nighat Seema1, Tasmia Bashir1, Ammarah Kanwal2 and Sidra Nisar Ahmed1
1Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
2Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Peshawar, Pakistan.
Accepted 14 April, 2017
Abstract
Ficus species have wide distribution and uses worldwide traditionally as medicine, vegetable, food, fodder and fuel wood etc. The flame atomic absorption spectrophotometer was employed for the estimation, conducted on four plant parts (leaves, bark, aerial roots and fruits) of 12 samples of eight medicinally important species of Ficus and nine samples of wild edible fruits of genus Ficus, collected from different locations within Pakistan. The results of the present study provide justification for the usage of these fruits in daily diet for nutrition as well as for medicinal usage and medicinal plants in the treatment of different diseases. The metal contents in the samples were found at different levels which play a vital role in cure of diseases. Toxic elements Cd and Pb were also found but at very low concentration. These results can give the importance about the wild edible fruits and used to set new standards for prescribing the dosage of the herbal drugs prepared from these plant materials in herbal remedies and in pharmaceutical companies.
Key words: Atomic absorption spectrometer (AAS), drugs, elements, Ficus, fruits, medicinal.
Paras Mazari, Mir Ajab Khan, Kiran Yasmin Khan*, Tasmia Bashir, Rabia Niamat, Nighat Seema, Mamoona Munir, Ammarah Kanwal and Sidra Nisar Ahmed, Hina Fazal
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