ISSN 2326-7267
Research Article
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (12), pp. 001-004, December, 2017. © International Scholars Journals
Full Length Research Paper
Analgesic activities of ethanolic extract of the root of Carpolobia lutea
Clement Jackson*, Herbert Mbagwu, Idongesit Jackson, Godwin Ekpe and Florence Etienam
Faculty of Pharmacy, University of Uyo, Uyo, Nigeria.
Accepted 15 March, 2017
Abstract
The analgesic activities of the aqueous extract of Carpolobia lutea was evaluated in mice and rats using the mouse writhing, tail flick and formalin induced pain tests. Analgesic studies were performed using three models; mouse writhing assay, formalin test and tail flick assay. The extract (1500 to 2500 mg/kg) and acetylsalicylic acid (100 mg/kg) produced a significant (P<0.05) inhibition of the second phase in the formalin pain model, while the antinoceptive effect was not produced in the first phase. The extract also showed a dose dependent inhibition of acetic acid induced abdominal writhings. The tail flick latency was not enhanced by the extract. Oral administration of the extract up to 2500 mg/kg did not produce any toxic effects in the acute toxicity studies in mice. The LD50 of the extract when administered orally was 3338.83 mg/kg. The data obtained shows that C. lutea posseses analgesic activity that is peripherally mediated.
Key words: Antinoceptive, Carpolobia lutea, LD50, pain model.
Idongesit Jackson, Herbert Mbagwu, Clement Jackson*, Godwin Ekpe and Florence Etienam
Page: 1 - 4
Research Article
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (12), pp. 001-005, December, 2017. © International Scholars Journals
Full Length Research Paper
Influence of the aryl substituent identity in 4-arylamino-3-nitrocoumarins on their antimicrobial activity
Vidoslav Dekić1, Niko Radulović2*, Rastko Vukićević3, Biljana Dekić1, Zorica Stojanović-Radić4 and Radosav Palić2
1Department of Chemistry, Faculty of Science and Mathematics, University of Priština, Lole Ribara 29, 38220 Kosovska, Mitrovica, Serbia.
2Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Višegradska 33, 18000 Niš, Serbia.
3Department of Chemistry, Faculty of Science, University of Kragujevac, R. Domanovića 12, 34000 Kragujevac, Serbia.
4Department of Biology and Ecology, Faculty of Science and Mathematics, University of Niš, Višegradska 33, 18000 Niš, Serbia.
Accepted 14 March, 2017
Abstract
Two new and six previously known coumarin derivatives with promising biological properties were synthesized in moderate to good yields by reaction of 4-chloro-3-nitro-coumarin and the appropriate arylamine in ethyl acetate in the presence of triethylamine. The synthesized compounds were evaluated for their in vitro antibacterial and antifungal activities against pathogenic strains. A correlation between the aryl substituent identity and antimicrobial activity was discussed.
Key words: 4-Arylamino-3-nitro-coumarins, synthesis, NMR spectroscopy, 4-chloro-3-nitro-coumarin, arylamines, antimicrobial activity.
Zorica Stojanović-Radić and Radosav Palić, Biljana Dekić, Vidoslav Dekić, Niko Radulović*, Rastko Vukićević
Page: 1 - 5
Research Article
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (12), pp. 001-009, December, 2017. © International Scholars Journals
Full Length Research Paper
Aconitine mediates connexin43 and PKC phosphorylation status in cultured ventricular myocytes of neonatal rats
Yan Liu1, Man Liang1, Shi-wei Zhang2, Lan Zhou3, Chuan-hong Zhu4 and Liang Liu5*
1Department of Forensic Medicine, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
2Zhongshan Hospital of Hubei Province, Wuhan 430033, China.
3Institute of Forensic Science, Bureau of Public Security for Jiangsu Province, Nanjing 210024, China.
4Wuhan Municipla Public Security Bureau, Wuhan 430029, China.
5Key Laboratory of Evidence Science (China University of Political Science and Law), Ministry of Education, Beijing 100040, China.
Accepted 25 April, 2017
Abstract
Aconitine, a strong poisonous type of alkaloid, has a pharmaceutical effect in stimulating the membranes of cardiomyocyte. However, other effects of aconitine on the Connexin43 (Cx43) and PKC expression on cardiomyocyte are unknown. In this study, we investigated whether aconitine also mediates the phosphorylation status of Cx43 and PKC in cultured ventricular myocytes of neonatal rats. The band intensity of phosphorylated Cx43 and nonphosphorylated Cx43 in cultured and aconitine-treated cardiomyocytes were determined by Western blot analysis. The changes in phosphorylation status occurring in PKC in cultures were revealed by quantitative immunofluorescent microscopy. A decreased band intensity (0.37±0.04) of phosphorylated Cx43 (P-Cx43) and a concomitant increased band intensity (3.56 ± 0.65) of nonphosphorylated Cx43 (NP-Cx43) were found, compared to the controls (1.00 for P-Cx43 and NP-Cx43). It also revealed that, after aconitine treatment, the amount of phosphorylated PKC (P-PKC ) decreased significantly. Similar changes were revealed in phosphorylation status occurring in PKC in the cultures under the same treatment conditions. These observations suggest that aconitine not only induces dephosphorylation of Cx43, but also alters expression of P-PKC in cultured cardiomyocytes.
Key words: Aconitine, cardiomyocyte, connexin 43 (Cx43), protein kinase C- (PKC ), protein phosphorylation.
Lan Zhou, Shi-wei Zhang, Chuan-hong Zhu and Liang Liu*, Yan Liu, Man Liang
Page: 1 - 9
Research Article
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (12), pp. 001-006, December, 2017. © International Scholars Journals
Full Length Research Paper
Antinociceptive activity of some 1,4-substituted piperidine derivatives using tail flick method in mice
Amirhossein Sakhteman1, Mohammad Sharifzadeh2, Alireza Moradi1, Hamid Nadri1, Kaveh Tabrizian2,3, Massoud Amanlou4, Ali Asadipour5, Kouros Divsalar5, Abbas Shafiee4 and Alireza Foroumadi4,5*
1Department of Medicinal Chemistry, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.
3Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran.
4Department of Medicinal Chemistry, Faculty of Pharmacy and Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran.
5Department of Medicinal Chemistry, Faculty of Pharmacy and Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Accepted 13 March, 2017
Abstract
We have examined the potential antinociceptive effects of some piperidine derivatives (6a-f), using tail flick method in mice. Morphine was used as positive control drug. The results showed that compound 6b, having a bromine atom at 4 position, was the most active agent tested. The activity of this compound at 50 mg/kg was comparable to morphine (3 mg/kg). Based on the similarities between our compounds and meperidine like structures such as fentanyl, a group of mice was treated with naloxone before administration of 6f. It was concluded that opioid receptors could be the dominant mechanism for the antinociceptive activity of these types of structures. Electrostatic map, distance analysis and superimposition between fentanyl and compound 6b were also studied.
Key words: Piperidine derivatives, antinociceptive activity, tail flick.
Hamid Nadri, Mohammad Sharifzadeh, Kouros Divsalar, Alireza Moradi, Amirhossein Sakhteman, Kaveh Tabrizian, Massoud Amanlou, Ali Asadipour, Abbas Shafiee and Alireza Foroumadi*
Page: 1 - 6
Research Article
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (11), pp. 001-005, November, 2017. © International Scholars Journals
Full Length Research Paper
A comparison study of Morven gold and Tander virginia cigarettes with respect to its spasmogenic, spasmolytic and chemical screening
Naveed Ullah1*, Mir Azam Khan1, Afzal Haq Asif2, Habib Ahmad3, Taimur Khan4, Taous Khan5 and Waqar Ahmad1
1Department of Pharmacy, University of Malakand, Chakdara Pakistan.
2Department of Pharmacology, Frontier Medical College, Abbottabad Pakistan.
3Department of Genetics, Hazara University, Mansehra Pakistan.
4Department of Psychiatry, Lady Reading Hospital Peshawar, Pakistan
5Department of Pharmacy, Comsats Institute of Information & Technology, Abbottabad, Pakistan
Accepted 17 March, 2017
Abstract
The ethanolic extracts derived from cigarettes (Morven gold and Tander virginia) were screened for chemicals, spasmogenic and spasmolytic activities. M. gold extract showed a strong relaxant activity that is 70% against KCl induced contractions while T. virginia was found to have a mild spasmolytic activity of (06%). Furthermore, a moderate spasmogenic effect of M. gold had being measured, while no measurable spasmogenic activity has been shown by the T. virginia. It can be concluded from the current study that Morven gold has a strong spamsmogenic and spasmolytic activity, while the Tander is not found to be so efficient in either case. The chemicals found in sufficient quantity in both the extracts were tannins, saponin and glycosides. Minute quantity of carbohydrates were also been noted in M. gold . The presence of alkaloids were also been noted in excess quantity in T. virginia and less amount in M. gold. Further studies are necessary to elucidate its exact mechanism of action.
Key words: Spasmogenic, spasmolytic, chemical screening, Morven gold, Tander virginia.
Afzal Haq Asif, Mir Azam Khan, Naveed Ullah*, Habib Ahmad, Taous Khan and Waqar Ahmad, Taimur Khan
Page: 1 - 5
Research Article
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (11), pp. 001-005, November, 2017. © International Scholars Journals
Full Length Research Paper
Resistance to erythromycin of Campylobacter jejuni and Campylobacter coli isolated from animals and humans
Z. Tambur1*, B. Miljkovic-Selimovic2, Z. Kulisic 3, D. Mirkovic1, R. Doder1 and Z. Stanimirovic3
1Military Medical Academy, Belgrade, Serbia.
2Medical Faculty, University of Nis, Nis, Serbia.
3Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia.
Accepted 15 July, 2017
Abstract
The sensititivity of thermophilic Campylobacter strains isolated from caecum of broiler chickens as well as caecum and colon of pigs and human stools, were tested against erythromycin. In 16 strains isolated in broiler chickens, resistance rate was found to be 12.50%. Three of 10 strains of Campylobacter jejuni and one of 6 strains of Campylobacter coli isolated from broiler chickens were resistant to erythromycin. In 15 strains of thermophilic Campylobacters isolated from pigs, resistance rate to erythromycin was 40.00%. Resistance was exhibited more often in C. coli (50.00%) as compared to C. jejuni (20.00%). In 24 strains isolated from humans, resistance was demonstrated at the rate of 12.50%. Out of 17 strains of C. jejuni isolated from humans, resistance was exhibited in 17.65% strains. None of 7 strains of C. coli isolated from humans exhibited resistance to erythromycin. Thermophilic campylobacters, especially C. coli isolated from pigs were more resistant to erythromycin than strains isolated from humans and broiler chickens. Therefore, a great attention should be directed to the macrolides monitoring in swine farming in order to prevent resistance in animals and its subsequent spread to human.
Key words: Campylobacter spp., resistance, erythromycin.
B. Miljkovic-Selimovic, D. Mirkovic, R. Doder and Z. Stanimirovic, Z. Tambur*, Z. Kulisic
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