ISSN 2326-7267
Research Article
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (8), pp. 001-008, August, 2017. © International Scholars Journals
Full Length Research Paper
Stability-indicating (liquid chromatographic) LC method for the determination of rifabutin in bulk drug and in pharmaceutical dosage form
Jaiprakash N. Sangshetti, Sachin Hingankar, Amol Waghule and Devanand B. Shinde*
Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, India.
Accepted 15 March, 2017
Abstract
A novel stability-indicating LC assay method was developed and validated for quantitative determination of Rifabutin in bulk drugs and in pharmaceutical dosage form in the presence of degradation products generated from forced degradation studies. An isocratic, reversed phase LC method was developed to separate the drug from the degradation products, using an Ace5-C18 (250 x 4.6 mm, 5 µm) column, and 50 mM ammonium acetate (pH- 4 by acetic acid) and acetonitrile (50:50v/v) as a mobile phase. The detection was carried out at the wavelength of 275 nm. The Rifabutin was subjected to stress conditions of hydrolysis (acid, base), oxidation, photolysis and thermal degradation. Degradation was observed for Rifabutin hydrolysis (acid, base), oxidation and photolysis conditions attempted. There is no degradation in thermal condition. The degradation products were well resolved from the main peak. The percentage recovery of Rifabutin ranged from (99.42 to 100.27%) in pharmaceutical dosage form. The developed method was validated with respect to linearity, accuracy (recovery), precision, specificity and robustness. The forced degradation studies prove the stability-indicating power of the method.
Key words: Rifabutin, column liquid chromatography, stability indicating method, validation.
Jaiprakash N. Sangshetti, Amol Waghule and Devanand B. Shinde*, Sachin Hingankar
Page: 1 - 8
Case Report
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (8), pp. 001-006, August, 2017. © International Scholars Journals
Case Report
Avoidable childhood death: A case of mismanagement and inappropriate medication use
Taha Nazir*, Syed Muzzamil Masood Zaidi, Ashfaq Ahmad and Tahir Aqeel
The University of Lahore – Islamabad Campus, 24 Jinnah Avenue, Islamabad, Pakistan.
Accepted 25 March, 2016
Abstract
Rational drug usage, clinical regulations and pharmaceutical care are important features of health practice. Thus; we have aimed case report to elaborate the clinical pharmaceutical services. A 3 year old girl, was presented in a hospital with minor burn that happened by spilt hot water on her wrist. She was in severe pain. The emergency ward staff applied an ointment. The doctor instructed injection Dormicum two times with short gape and then 3rd injection of Pavulon (Pancuronium). Patient went silent and started losing consciousness and eventually passed away. In view of the above facts and circumstances the inquiry board and judiciary took action against the negligence, mishandling. While; going through the investigation the inquiry board did not bothered the channel through which this injection came without proper check that is therapeutical drug monitoring, prescription reviewing, bio-safety and pharmaceutical evaluation. Furthermore, it is not a single death but representation of so many casualties because of the wrong medications. The medial practice needs some more legal, professional and principled restrictions to make sure the safe and correct therapy plans. The clinical legislation, drug rules and medical practice seriously need the attention of judiciary, society and leadership to assure the safety of precious live.
Key words: Wrong medication, medical malpractice, professional negligence, pharmaceutical services, Suo-moto notice of chief justice of high court.
Taha Nazir*, Ashfaq Ahmad and Tahir Aqeel, Syed Muzzamil Masood Zaidi
Page: 1 - 6
Research Article
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (7), pp. 001-005, July, 2017. © International Scholars Journals
Full Length Research Paper
Screening for anti-typhoid activity of some medicinal plants used in traditional medicine in Ebonyi state, Nigeria
Iroha I. R.1*, ILang D. C.1, Ayogu T. E.2, Oji A. E.1 and Ugbo E. C.1
1Department of Applied Microbiology, Faculty of Biological Sciences, Ebonyi State University, P. M. B. 053, Abakaliki Ebonyi State, Nigeria.
2Department of Food Technology, Institute of Management and technology, Enugu, P. M. B. 01079, Enugu State, Nigeria.
Accepted 11 April, 2017
Abstract
Ethanol, hot and cold crude water extracts of five medicinal plants namely Vitex doniana (root) , Cassia tora (Leaf), Alstonia boonei (bark), Stachytarpheta jamaicensis (leaf), and Carica papaya (leaf) used as traditionally medicine for anti Salmonella typhi activity in Ebonyi state were evaluated. These plants were screened in-vitro for anti-typhoid activity against 10 clinically selected isolates of S. typhi using the hole-plate diffusion method. The Minimum inhibitory concentration (MIC) of Ethanol, hot and cold water extract of each herbal plant was determined by broth dilution method. Ethanol extracts of Vitex doniana exhibited anti-typhoid activity against 9(90%) of the test organisms, A. boonei exhibited activity against 8(80%) of the test organisms, C. papaya against 2(20%), C. tora against 6(60%), and S. jamaicensis against 6(60%). Hot water extract of Vitex doniana showed anti-typhoid activity against 7(70%) of the test organisms, A. boonei against 9(90%), C. papaya against 1(10%), C. tora against 8(80%) and S. jamaicensis against 7(70%). Cold water extract of V. doniana, had anti-typhoid activity against 6(60%) of the test organisms, A. boonei, against 6(60%), C. papaya against 0(0%), C. tora against 6(60%) and S. jamaicensis against 4(40%). MIC of ethanol, hot and cold water extracts of V. doniana, A. boonei, C. papaya, C. tora and S. jamaicensis, fall within 0.4 -128, 0.8 -128, 64 -128, 32 – 128 and 32 – 128. MIC of hot water extracts were within 16 -128, 0.8 – 128, 128 -512, 0.8 – 512 and 0.8 – 128 while MIC of cold water extract are within 64 – 128, 64 – 512, 64 – 512, 64 – 512 and 128-512 respectively. Our findings showed that ethanol and hot water extracts of V. doniana and A. boonei had the best anti-typhoid activity followed by C. tora and S. jamaicensis while C. papaya showed no activity.
Key words: Salmonella typhi, medicinal plants extracts, anti-typhoid activity, minimum inhibitory concentration.
Oji A. E. and Ugbo E. C., Ayogu T. E., ILang D. C., Iroha I. R.*
Page: 1 - 5
Research Article
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (7), pp. 001-009, July, 2017. © International Scholars Journals
Full Length Research Paper
Activity induced by dihydrotestosterone-dihydropyrimidine derivative on perfusion pressure and coronary resistance in isolated rat heart
Figueroa-Valverde Lauro1*, Díaz-Cedillo Francisco2, Lopez-Ramos Ma1, Garcia-Cervera Elodia1 and Quijano A. Karen1
1Laboratorio de Investigación en Ciencias Biologicas y Farmacoquímica de la Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Campeche, Av. Agustín Melgar entre calle 20 y Juan de la Barrera, Col Buenavista C.P. 24039 Campeche Cam., México.
2Laboratorio de Química Orgánica de la Esc. Nal. de Ciencias Biológicas del Instituto Politécnico Nacional. Prol. Carpio y Plan de Ayala s/n Col. Santo Tomas, México, D.F. C.P. 11340.
Accepted 10 March, 2017
Abstract
Experimental and clinical studies suggest that dihydrotestosterone can be associated with changes in blood pressure. In this work, the effects induced by dihydrotestosterone and dihydrotestosterone-dihydropyrimidine on perfusion pressure and coronary resistance were evaluated, in isolated rat heart using the Langendorff flow model. Additionally, the molecular mechanism involved in the activity exerted by dihydrotestosterone-derivative was characterized. The results showed that dihydrotestosterone- dihydropyrimidine [10-9 mM] significantly increase the perfusion pressure (p = 0.005) and coronary resistance (p = 0.006) in isolated rat heart. Additionally, the activity exerted by dihydrotestosterone-dihydropyrimidine on perfusion pressure [109 to 10-4 mM] was blocked in the presence of nefidepine [10-6 mM].These data suggest that activity induced by dihydrotestosterone-derivative on perfusion pressure and coronary resistance is dependent upon its chemical structure. This phenomenon possibly involves the L-type calcium channel activation through a non-genomic molecular mechanism.
Key words: Dihydrotestosterone, perfusion pressure, vascular resistance.
Figueroa-Valverde Lauro*, DÃaz-Cedillo Francisco, Garcia-Cervera Elodia and Quijano A. Karen, Lopez-Ramos Ma
Page: 1 - 9
Research Article
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (6), pp. 001-010, June, 2017. © International Scholars Journals
Full Length Research Paper
Anticarcinogenic effect of turmeric on processed food products containing acrylamide
Akshaya Pandey1, Prateek Garg1 and R. Balaji Raja2*
1Department of Biotechnology, SRM University, Kattankulathur – 603203, Tamil Nadu, India.
2Department of Biotechnology, National Institute of Technology, Raipur – 492001, Chhattisgarh, India.
Accepted 13 March, 2017
Abstract
In present scenario Acrylamide concentration in processed food products have become a very serious health issue. The World Health Organization (WHO) and The Scientific Committee for Food (SCF) of the European Union also confirmed this concern. In laboratory scale, it was found that Acrylamide causes tumors in animals. It is even present in processed food free of microbes indicating the absence of microbial role in Acrylamide formation. This study was aimed to demonstrate the anti-carcinogenic effect of Acrylamide in the processed food products available in open market. In order to determine the acrylamide concentration in three processed food brands GC-MS technique was employed. Turmeric was found to bring about the anti-carcinogenic effect and lower down the acrylamide concentration in Aloo Paratha. No profound concentration of acrylamide was found in other two processed food products taken in for study. The processed food is found to be a Health hazard to the consumer and discretion of usage could minimize its impact.
Key words: Processed food, GC-MS, acrylamide, anti-carcinogen, turmeric.
Akshaya Pandey, Prateek Garg and R. Balaji Raja*
Page: 1 - 10
Research Article
International Journal of Pharmacy and Pharmacology ISSN: 2326-7267 Vol. 6 (6), pp. 001-007, June, 2017. © International Scholars Journals
Full Length Research Paper
Impact of saffron as an anti-cancer and anti-tumor herb
Siavash Hosseinpour Chermahini1*, Fadzilah Adibah Abd. Majid1, Mohamad Roji Sarmidi1,2, Ehsan Taghizadeh3 and Saleh Salehnezhad4
1Department of Bioprocess Engineering, Faculty of Chemical Engineering, University Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia.
2Research Alliance (Biotechnology), Chemical Engineering Pilot Plant (CEPP), University Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia.
3Department of Pharmacy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
4Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Accepted 19 February, 2017
Abstract
Cancer continues to represent the largest cause of mortality in the world and claims over 6 million lives each year. An extremely promising strategy for cancer prevention today is chemoprevention, which is defined as the use of synthetic or natural agents (alone or in combination) to block the development of cancer in human beings. Plants, vegetables, herbs and spices used in folk and traditional medicine have been accepted currently as one of the main sources of cancer chemopreventive drug discovery and development. This review gives an overview from one of this herbs and spices that is saffron. The chemical composition of saffron has attracted the interest of several research groups during the last decades, and among the estimated more than 150 volatile and several nonvolatile compounds of saffron, approximately 40 – 50 constituents have already been identified. Oral administration of saffron extract inhibited the growth of mouse tumors that were derived from three different kinds of cancer cells and significantly increased the life spans of treated tumor-bearing mice.
Key words: Chemoprevention, volatile, tumor-bearing.
Ehsan Taghizadeh and Saleh Salehnezhad, Fadzilah Adibah Abd. Majid, Mohamad Roji Sarmidi, Siavash Hosseinpour Chermahini*
Page: 1 - 7